ArticlePLoS pathogens2014
Functional characterisation of germinant receptors in Clostridium botulinum and Clostridium sporogenes presents novel insights into spore germination systems.
Article in PLoS pathogens, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Clostridium septicum manifests a bile salt germinant response mediated by Clostridioides difficile csp gene orthologs.Communications biology · 2024Article
- SpoIVA is an essential morphogenetic protein for the formation of heat- and lysozyme-resistant spores inFrontiers in microbiology · 2024Article
- A design of experiments screen reveals that Clostridium novyi-NT spore germinant sensing is stereoflexible for valine and its analogs.Communications biology · 2023Article
- Potential Risk of Botulinum Neurotoxin -producing Clostridia Occurrence in Canned Fish.Journal of veterinary research · 2022Article
- Mechanisms and Applications of Bacterial Sporulation and Germination in the Intestine.International journal of molecular sciences · 2022Review
- Occurrence of genes encoding spore germination inMicrobial genomics · 2022Article
- Dormant spores sense amino acids through the B subunits of their germination receptors.Nature communications · 2021Article
- Efficient Secretion of Murine IL-2 From an Attenuated Strain ofFrontiers in microbiology · 2021Article
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- The orphan germinant receptor protein GerXAO (but not GerX3b) is essential for L-alanine induced germination in Clostridium botulinum Group II.Scientific reports · 2018Article
- Superdormant Spores as a Hurdle for Gentle Germination-Inactivation Based Spore Control Strategies.Frontiers in microbiology · 2018Article
- Canonical germinant receptor is dispensable for spore germination in Clostridium botulinum group II strain NCTC 11219.Scientific reports · 2017Article
- A Novel Technique for Sterilization Using a Power Self-Regulated Single-Mode Microwave Cavity.Sensors (Basel, Switzerland) · 2017Article
- Analysis of the Germination of IndividualFrontiers in microbiology · 2017Article
- An Integrative Approach to Computational Modelling of the Gene Regulatory Network Controlling Clostridium botulinum Type A1 Toxin Production.PLoS computational biology · 2016Article
- Germinants and Their Receptors in Clostridia.Journal of bacteriology · 2016Review
- Impact ofCurrent opinion in food science · 2016Review
- Draft Genome Sequence of Clostridium sporogenes Strain UC9000 Isolated from Raw Milk.Genome announcements · 2016Article
- Diversity of the Germination Apparatus inFrontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Clostridium botulinum is a dangerous pathogen that forms the highly potent botulinum toxin, which when ingested causes a deadly neuroparalytic disease. The closely related Clostridium sporogenes is occasionally pathogenic, frequently associated with food spoilage and regarded as the non-toxigenic equivalent of Group I C. botulinum. Both species form highly resistant spores that are ubiquitous in the environment and which, under favourable growth conditions germinate to produce vegetative cells. To improve the control of botulinum neurotoxin-forming clostridia, it is imperative to comprehend the mechanisms by which spores germinate. Germination is initiated following the recognition of small molecules (germinants) by a specific germinant receptor (GR) located in the spore inner membrane. The present study precisely defines clostridial GRs, germinants and co-germinants. Group I C. botulinum ATCC3502 contains two tricistronic and one pentacistronic GR operons, while C. sporogenes ATCC15579 has three tricistronic and one tetracistronic GR operons. Insertional knockout mutants, allied with characterisation of recombinant GRs shows for the first time that amino acid stimulated germination in C. botulinum requires two tri-cistronic encoded GRs which act in synergy and cannot function individually. Spore germination in C. sporogenes requires one tri-cistronic GR. Two other GRs form part of a complex involved in controlling the rate of amino-acid stimulated germination. The suitability of using C. sporogenes as a substitute for C. botulinum in germination studies and food challenge tests is discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.