ArticleEvoDevo2014
The role of a lens survival pathway including sox2 and αA-crystallin in the evolution of cavefish eye degeneration.
Article in EvoDevo, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 53 citations in OpenAlex.
- Hypoxia-sonic hedgehog axis as a driver of primitive hematopoiesis development and evolution in cavefish.Developmental biology · 2024Article
- Natural reversal of cavefish heart asymmetry is controlled by Sonic Hedgehog effects on the left-right organizer.Development (Cambridge, England) · 2024Article
- Metabolic shift toward ketosis in asocial cavefish increases social-like affinity.BMC biology · 2023Article
- Trait Loss in Evolution: What Cavefish Have Taught Us about Mechanisms Underlying Eye Regression.Integrative and comparative biology · 2023Review
- Discovery of putative long non-coding RNAs expressed in the eyes of Astyanax mexicanus (Actinopterygii: Characidae).Scientific reports · 2023Article
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- Article
- Maternal control of visceral asymmetry evolution in Astyanax cavefish.Scientific reports · 2021Article
- A hypomorphic cystathionine ß-synthase gene contributes to cavefish eye loss by disrupting optic vasculature.Nature communications · 2020Article
- Article
- Review
- Eye Degeneration and Loss of otx5b Expression in the Cavefish Sinocyclocheilus tileihornes.Journal of molecular evolution · 2019Article
- Maternal genetic effects in Astyanax cavefish development.Developmental biology · 2018Article
- An epigenetic mechanism for cavefish eye degeneration.Nature ecology & evolution · 2018Article
- Article
- Cavefish and the basis for eye loss.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Lens apoptosis in the Astyanax blind cavefish is not triggered by its small size or defects in morphogenesis.PloS one · 2017Article
- The Comparative Organismal Approach in Evolutionary Developmental Biology: Insights from Ascidians and Cavefish.Current topics in developmental biology · 2016Review
- The rise of Astyanax cavefish.Developmental dynamics : an official publication of the American Association of Anatomists · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
backgroundThe teleost Astyanax mexicanus is a single species consisting of eyed surface-dwelling (surface fish) and blind cave-dwelling (cavefish) morphs. Cavefish eyes are lost through apoptosis of the lens, which in turn promotes the degeneration of other optic tissues. The αA-crystallin (αA-crys) gene is strongly downregulated in the cavefish lens and is located in a genomic region (QTL) responsible for eye loss. Therefore, αA-crys has been proposed as a candidate for regulating cavefish eye degeneration. The purpose of this study was to determine the mechanism of αA-crys downregulation and its role in cavefish eye degeneration.
resultsThe involvement of αA-crys in eye degeneration was confirmed by knocking down its expression in surface fish, which led to apoptosis of the lens. The underlying reason for αA-crys downregulation in cavefish was investigated by comparing genomic αA-crys DNA sequences in surface fish and cavefish, however, no obvious cis-regulatory factors were discovered. Furthermore, the cavefish αA-crys allele is expressed in surface fish x cavefish F1 hybrids, indicating that evolutionary changes in upstream genes are most likely responsible for αA-crys downregulation. In other species, Sox2 is one of the transcription factors that regulate lens crystallin genes during eye development. Determination of sox2 expression patterns during surface fish and cavefish development showed that sox2 is specifically downregulated in the cavefish lens. The upstream regulatory function of Sox2 was demonstrated by knockdown in surface fish, which abolished αA-crys expression and induced lens apoptosis.
conclusionsThe results suggest that αA-crys is required for normal eye development in cavefish via suppression of lens apoptosis. The regulatory changes involved in αA-crys downregulation in cavefish are in trans-acting factors rather than cis-acting mutations in the αA-crys gene. Therefore, αA-crys is unlikely to be the mutated gene(s) associated with an Astyanax eye QTL. The results reveal a genetic pathway leading from sox2 to αA-crys that is required for survival of the lens in Astyanax surface fish. Defects in this pathway may be involved in lens apoptosis and thus a cause of cavefish eye degeneration.
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