ArticleJournal of clinical microbiology2014
PCR-based detection of Toxoplasma gondii DNA in blood and ocular samples for diagnosis of ocular toxoplasmosis.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 68 citations in OpenAlex.
- Evaluation of Laboratory Techniques for the Diagnosis of Leptospira-Associated Equine Recurrent Uveitis (ERU) With Focus on the Goldmann-Witmer Coefficient.Veterinary ophthalmology · 2026Article
- A novel TaqMan probe-based pentaplex qPCR assay for the simultaneous detection of five pathogenic protozoans.Scientific reports · 2025Article
- Trend in serological and molecular diagnostic methods for Toxoplasma gondii infection.European journal of medical research · 2024Review
- Molecular diagnosis of human toxoplasmosis: the state of the art.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Review
- Evaluation of species-specific polyclonal antibodies to detect and differentiate betweenJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- Considerations in the management of ocular toxoplasmosis in pregnancy: a review of literature.Eye (London, England) · 2024Review
- Standardization of the PCR Technique for the Detection of the Toxoplasma gondii B1 Gene in Meat and Water Samples and Cloning of the Product for Use as Control.Acta parasitologica · 2022Article
- Utility of blood as the clinical specimen for the diagnosis of ocular toxoplasmosis using uracil DNA glycosylase-supplemented loop-mediated isothermal amplification and real-time polymerase chain reaction assays based on REP-529 sequence and B1 gene.BMC infectious diseases · 2022Article
- Ocular toxoplasmosis: a review of the current diagnostic and therapeutic approaches.International ophthalmology · 2022Review
- Protein expression of the tear film of domestic cats before and after inoculation with Toxoplasma gondii.BMC veterinary research · 2021Article
- Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Ocular Infections.Clinical microbiology reviews · 2021Review
- Study of toxoplasmosis and toxocariasis in patients suffering from ophthalmic disorders using serological and molecular methods.International ophthalmology · 2020Article
- Evaluation of a PCR assay for diagnosis of toxoplasmosis in serum and peripheral blood mononuclear cell among HIV/AIDS patients.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2020Article
- Development of a Novel Protocol Based on Blood Clot to Improve the Sensitivity of qPCR Detection ofThe American journal of tropical medicine and hygiene · 2019Article
- Immunological Molecular Responses of Human Retinal Pigment Epithelial Cells to Infection WithFrontiers in immunology · 2019Article
- Toxoplasma gondii: Laboratory Maintenance and Growth.Current protocols in microbiology · 2017Article
- Evaluating the presence of Toxoplasma gondii in peripheral blood of patients with diverse forms of uveitis.International ophthalmology · 2017Article
- Performance Testing of PCR Assay in Blood Samples for the Diagnosis of Toxoplasmic Encephalitis in AIDS Patients from the French Departments of America and Genetic Diversity of Toxoplasma gondii: A Prospective and Multicentric Study.PLoS neglected tropical diseases · 2016Article
- A Brazilian report using serological and molecular diagnosis to monitoring acute ocular toxoplasmosis.BMC research notes · 2015Article
- Ocular Toxoplasmosis in a Tertiary Referral Center in Sydney Australia-Clinical Features, Treatment, and Prognosis.Asia-Pacific journal of ophthalmology (Philadelphia, Pa.)Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PCR detection of Toxoplasma gondii in blood has been suggested as a possibly efficient method for the diagnosis of ocular toxoplasmosis (OT) and furthermore for genotyping the strain involved in the disease. To assess this hypothesis, we performed PCR with 121 peripheral blood samples from 104 patients showing clinical and/or biological evidence of ocular toxoplasmosis and from 284 (258 patients) controls. We tested 2 different extraction protocols, using either 200 μl (small volume) or 2 ml (large volume) of whole blood. Sensitivity was poor, i.e., 4.1% and 25% for the small- and large-volume extractions, respectively. In comparison, PCR with ocular samples yielded 35.9% sensitivity, while immunoblotting and calculation of the Goldmann-Witmer coefficient yielded 47.6% and 72.3% sensitivities, respectively. Performing these three methods together provided 89.4% sensitivity. Whatever the origin of the sample (ocular or blood), PCR provided higher sensitivity for immunocompromised patients than for their immunocompetent counterparts. Consequently, PCR detection of Toxoplasma gondii in blood samples cannot currently be considered a sufficient tool for the diagnosis of OT, and ocular sampling remains necessary for the biological diagnosis of OT.
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