ArticleChembiochem : a European journal of chemical biology2014
Recombinase-based isothermal amplification of nucleic acids with self-avoiding molecular recognition systems (SAMRS).
Article in Chembiochem : a European journal of chemical biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Annexing isothermal nucleotide amplification enables probe-guided amplification and direct detection at ambient temperature.Nature communications · 2026Article
- Development of an isothermal point of care method for detection of human papilloma virus (HPV).Aspects of molecular medicine · 2026Article
- Rapid detection of Seneca Valley virus based on reverse transcription recombinase polymerase amplification and CRISPR-Cas13a.Virulence · 2025Article
- Multiplexed Salivary miRNA Quantification for Predicting Severe COVID-19 Symptoms in Children Using Ligation-RPA Amplification Assay.ACS sensors · 2025Article
- Development of A Novel EvaGreen-Dye Based Recombinase Aided Amplification Assay Using Self-Avoiding Molecular Recognition System Primers.China CDC weekly · 2025Article
- Multiplex CRISPR-Cas Assay for Rapid, Isothermal and Visual Detection of White Spot Syndrome Virus (WSSV) and Enterocytozoon hepatopenaei (EHP) in Penaeid Shrimp.Journal of fish diseases · 2025Article
- Advances in Virus Detection Techniques Based on Recombinant Polymerase Amplification.Molecules (Basel, Switzerland) · 2024Review
- Recombinase-based amplification coupled with lateral flow chromatography for the specific and sensitive detection and identification ofFrontiers in microbiology · 2024Article
- Application of recombinase polymerase amplification with lateral flow assay to pathogen point-of-care diagnosis.Frontiers in cellular and infection microbiology · 2024Review
- An Accurate, Rapid and Cost-Effective Method for T-nos Detection Based on CRISPR/Cas12a.Foods (Basel, Switzerland) · 2023Article
- Rapid isothermal point-of-care test for screening of SARS-CoV-2 (COVID-19).Aspects of molecular medicine · 2023Review
- Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics.Nature communications · 2022Article
- Malaria diagnostic update: From conventional to advanced method.Journal of clinical laboratory analysis · 2022Review
- Graphene oxide and self-avoiding molecular recognition systems-assisted recombinase polymerase amplification coupled with lateral flow bioassay for nucleic acid detection.Mikrochimica acta · 2020Article
- Rapid Authentication of Ginkgo biloba Herbal Products Using the Recombinase Polymerase Amplification Assay.Scientific reports · 2018Article
- Recombinase polymerase amplification: Basics, applications and recent advances.Trends in analytical chemistry : TRAC · 2018Review
- Towards Multiplex Molecular Diagnosis-A Review of Microfluidic Genomics Technologies.Micromachines · 2017Review
- Alternative Watson-Crick Synthetic Genetic Systems.Cold Spring Harbor perspectives in biology · 2016Review
- Standard and AEGIS nicking molecular beacons detect amplicons from the Middle East respiratory syndrome coronavirus.Journal of virological methods · 2016Article
- Helicase-Dependent Isothermal Amplification of DNA and RNA by Using Self-Avoiding Molecular Recognition Systems.Chembiochem : a European journal of chemical biology · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recombinase polymerase amplification (RPA) is an isothermal method to amplify nucleic acid sequences without the temperature cycling that classical PCR uses. Instead of using heat to denature the DNA duplex, RPA uses recombination enzymes to swap single-stranded primers into the duplex DNA product; these are then extended using a strand-displacing polymerase to complete the cycle. Because RPA runs at low temperatures, it never forces the system to recreate base-pairs following Watson-Crick rules, and therefore it produces undesired products that impede the amplification of the desired product, complicating downstream analysis. Herein, we show that most of these undesired side products can be avoided if the primers contain components of a self-avoiding molecular recognition system (SAMRS). Given the precision that is necessary in the recombination systems for them to function biologically, it is surprising that they accept SAMRS. SAMRS-RPA is expected to be a powerful tool within the range of amplification techniques available to scientists.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.