ArticleBiology letters2014
DNA metabarcoding and the cytochrome c oxidase subunit I marker: not a perfect match.
Article in Biology letters, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 157 papers.
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Who cites it
157 citing papers in PubMed.
- Diet Metabarcoding Reveals Correlations Between the Consumption of Slugs and Hepatic Toxicants in European Hedgehogs (Erinaceus europaeus).Integrative zoology · 2026Article
- A Roadmap for Using Hybridisation Capture-Based Target Enrichment of Ancient Environmental DNA in Palaeoecology.Molecular ecology resources · 2026Review
- A validated DNA metabarcoding approach for routine authentication of processed bivalves.Food chemistry. Molecular sciences · 2026Article
- MtCOI-based genetic diversity and population structure of the White-Backed Planthopper, Sogatella furcifera Horváth across major rice ecosystems of India.Molecular biology reports · 2026Article
- Validating COI eDNA Metabarcoding Primers for Detection of Subterranean Fauna.Molecular ecology resources · 2026Article
- Comparative Multi-Marker Environmental DNA Metabarcoding of Marine Metazoan Communities: Water vs. Sediment.Molecular ecology resources · 2026Article
- Mitogenomic and Metabarcoding Resources for the Study and Conservation of Keystone Neotropical Raptors.Ecology and evolution · 2026Article
- Unveiling Vertebrate Biodiversity in Arid and Semi-Arid Terrestrial Ecosystems Through eDNA Metabarcoding at Savanna Waterholes.Evolutionary applications · 2026Article
- Genetic structure and haplotype diversity in Indian populations of brown plant hopper, Nilaparvata lugens stal using mtCOI.Scientific reports · 2026Article
- Article
- Metabarcoding and targeted barcoding can enhance Norwegian Continental Shelf macrofauna species inventories.PeerJ · 2026Article
- Characterization of the OmnivorousEcology and evolution · 2026Article
- The shared performance of different COI regions increases the niche breadth detectable from bat guano eDNA.Genetics and molecular biology · 2026Article
- Joint morphological and DNA metabarcoding gut content analysis revealed that adult body size, but not biological sex, influences variance in dietary composition within and among cunner wrasse (BMC marine science · 2026Article
- Dietary DNA Metabarcoding From Animal Fecal Samples.Current protocols · 2026Article
- Can Amplicon Sequencing Be Replaced by Metagenomics for Biodiversity Inventories?Molecular ecology resources · 2025Article
- Review
- A DNA Barcode Inventory of Austrian Dragonfly and Damselfly (Insecta: Odonata) Species.Insects · 2025Article
- Structure and Composition of Prey Communities Associated with Malaysian Mahseer,Tropical life sciences research · 2025Article
- Mock Plant Communities and a Large Mammal Case Study Reveal ITS2 Primer Bias Against Graminoids.Ecology and evolution · 2025Article
97 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA metabarcoding enables efficient characterization of species composition in environmental DNA or bulk biodiversity samples, and this approach is making significant and unique contributions in the field of ecology. In metabarcoding of animals, the cytochrome c oxidase subunit I (COI) gene is frequently used as the marker of choice because no other genetic region can be found in taxonomically verified databases with sequences covering so many taxa. However, the accuracy of metabarcoding datasets is dependent on recovery of the targeted taxa using conserved amplification primers. We argue that COI does not contain suitably conserved regions for most amplicon-based metabarcoding applications. Marker selection deserves increased scrutiny and available marker choices should be broadened in order to maximize potential in this exciting field of research.
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