ArticleGenetics2014
High-efficiency genome editing and allele replacement in prototrophic and wild strains of Saccharomyces.
Article in Genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Estimating the distribution of fitness effects of loss of heterozygosity (LOH) events using an engineered library of Saccharomyces cerevisiae.PLoS genetics · 2026Article
- Simultaneous multigene integration in Aspergillus fumigatus using CRISPR/Cas9 and endogenous counter-selectable markers.Journal of biological engineering · 2025Article
- Epistasis and cryptic QTL identified using modified bulk segregant analysis of copper resistance in budding yeast.Genetics · 2025Article
- Epistasis and cryptic QTL identified using modified bulk segregant analysis of copper resistance in budding yeast.bioRxiv : the preprint server for biology · 2024Article
- Spontaneous single-nucleotide substitutions and microsatellite mutations have distinct distributions of fitness effects.PLoS biology · 2024Article
- Engineering transcriptional regulation of pentose metabolism in Rhodosporidium toruloides for improved conversion of xylose to bioproducts.Microbial cell factories · 2023Article
- The rate of spontaneous mutations in yeast deficient for MutSβ function.G3 (Bethesda, Md.) · 2023Article
- A Saccharomyces eubayanus haploid resource for research studies.Scientific reports · 2022Article
- Engineering of Synthetic Transcriptional Switches in Yeast.Life (Basel, Switzerland) · 2022Review
- Current Therapy and Therapeutic Targets for Microsporidiosis.Frontiers in microbiology · 2022Review
- Synthetic hybrids of six yeast species.Nature communications · 2020Article
- DNA variants affecting the expression of numerous genes in trans have diverse mechanisms of action and evolutionary histories.PLoS genetics · 2019Article
- A Seamless Gene Deletion Method and Its Application for Regulation of Higher Alcohols and Ester in BaijiuBioMed research international · 2019Article
- Mitochondrial DNA and temperature tolerance in lager yeasts.Science advances · 2019Article
- Repeated Cis-Regulatory Tuning of a Metabolic Bottleneck Gene during Evolution.Molecular biology and evolution · 2018Article
- Genome-wide association across Saccharomyces cerevisiae strains reveals substantial variation in underlying gene requirements for toxin tolerance.PLoS genetics · 2018Article
- CRISPR/Cas system for yeast genome engineering: advances and applications.FEMS yeast research · 2017Review
- Hybridization and adaptive evolution of diverseBiotechnology for biofuels · 2017Article
- Ongoing resolution of duplicate gene functions shapes the diversification of a metabolic network.eLife · 2016Article
- Horizontally acquired genes in early-diverging pathogenic fungi enable the use of host nucleosides and nucleotides.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Current genome editing techniques available for Saccharomyces yeast species rely on auxotrophic markers, limiting their use in wild and industrial strains and species. Taking advantage of the ancient loss of thymidine kinase in the fungal kingdom, we have developed the herpes simplex virus thymidine kinase gene as a selectable and counterselectable marker that forms the core of novel genome engineering tools called the H: aploid E: ngineering and R: eplacement P: rotocol (HERP) cassettes. Here we show that these cassettes allow a researcher to rapidly generate heterogeneous populations of cells with thousands of independent chromosomal allele replacements using mixed PCR products. We further show that the high efficiency of this approach enables the simultaneous replacement of both alleles in diploid cells. Using these new techniques, many of the most powerful yeast genetic manipulation strategies are now available in wild, industrial, and other prototrophic strains from across the diverse Saccharomyces genus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.