ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2014
Combined use of bulked segregant analysis and microarrays reveals SNP markers pinpointing a major QTL for resistance to Phytophthora capsici in pepper.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 66 citations in OpenAlex.
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- Glycoside Hydrolase CaGH17-12 Contributes to Phytophthora Blight Resistance by Activating the Production of Reactive Oxygen Species and Jasmonic Acid Signalling in Pepper.Molecular plant pathology · 2026Article
- Transcriptome profiling reveals tissue-wide gene expression in chili pepper (Frontiers in plant science · 2026Article
- CaSBP12 is implicated in pepper's defense resistance to Phytophthora capsici infection associated with the SA signaling pathway.BMC plant biology · 2025Article
- Insights into the genetic architecture of Phytophthora capsici root rot resistance in chile pepper (Capsicum spp.) from multi-locus genome-wide association study.BMC plant biology · 2024Article
- Exploring Disease Resistance in Pepper (Plants (Basel, Switzerland) · 2024Article
- Development and Application of a Cleaved Amplified Polymorphic Sequence Marker (Plants (Basel, Switzerland) · 2023Article
- Development and validation of KASP markers for resistance toMolecular breeding : new strategies in plant improvement · 2023Article
- A reference-anchored oat linkage map reveals quantitative trait loci conferring adult plant resistance to crown rust (Puccinia coronata f. sp. avenae).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Comparative Transcriptome and Metabolome Analysis of Resistant and SusceptibleFrontiers in plant science · 2022Article
- Genome-Wide Association Study of Resistance toFrontiers in plant science · 2022Article
- Genomic regions and candidate genes linked with Phytophthora capsici root rot resistance in chile pepper (Capsicum annuum L.).BMC plant biology · 2021Article
- Biotechnological approaches in management of oomycetes diseases.3 Biotech · 2021Review
- A combined BSA-Seq and linkage mapping approach identifies genomic regions associated with Phytophthora root and crown rot resistance in squash.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Comparative Genomic Analysis Reveals Genetic Variation and Adaptive Evolution in the Pathogenicity-Related Genes ofFrontiers in microbiology · 2021Article
- Overview of Biotic Stresses in Pepper (International journal of molecular sciences · 2020Review
- Fine mapping and identification of the leaf shape gene BoFL in ornamental kale.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- QTL Mapping of Kernel Traits and Validation of a Major QTL for Kernel Length-Width Ratio Using SNP and Bulked Segregant Analysis in Wheat.Scientific reports · 2020Article
- Target sequencing reveals genetic diversity, population structure, core-SNP markers, and fruit shape-associated loci in pepper varieties.BMC plant biology · 2019Article
- Identifying candidate genes for Phytophthora capsici resistance in pepper (Capsicum annuum) via genotyping-by-sequencing-based QTL mapping and genome-wide association study.Scientific reports · 2019Article
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Authors and funding
10 authors at 4 institutions in 3 countries.
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Abstract
key messageBulked segregant analysis (BSA) using Affymetrix GeneChips revealed candidate genes underlying the major QTL for Phytophthora capsici resistance in Capsicum. Using the candidate genes, reliable markers for Phytophthora resistance were developed and validated. Phytophthora capsici L. is one of the most destructive pathogens of pepper (Capsicum spp.). Resistance of pepper against P. capsici is controlled by quantitative trait loci (QTL), including a major QTL on chromosome 5 that is the predominant contributor to resistance. Here, to maximize the effect of this QTL and study its underlying genes, an F2 population and recombinant inbred lines were inoculated with P. capsici strain JHAI1-7 zoospores at a low concentration (3 × 10(3)/mL). Resistance phenotype segregation ratios for the populations fit a 3:1 and 1:1 (resistant:susceptible) segregation model, respectively, consistent with a single dominant gene model. Bulked segregant analysis (BSA) using Affymetrix GeneChips revealed a single position polymorphism (SPP) marker mapping to the major QTL. When this SPP marker (Phyto5SAR) together with other SNP markers located on chromosome 5 was used to confirm the position of the major QTL, Phyto5SAR showed the highest LOD value at the QTL. A scaffold sequence (scaffold194) containing Phyto5SAR was identified from the C. annuum genome database. The scaffold contained two putative NBS-LRR genes and one SAR 8.2A gene as candidates for contributing to P. capsici resistance. Markers linked to these genes were developed and validated by testing 100 F1 commercial cultivars. Among the markers, Phyto5NBS1 showed about 90% accuracy in predicting resistance phenotypes to a low-virulence P. capsici isolate. These results suggest that Phyto5NBS1 is a reliable marker for P. capsici resistance and can be used for identification of a gene(s) underlying the major QTL on chromosome 5.
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