ArticlePloS one2014
Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in L929 cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 55 citations in OpenAlex.
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- Morphology, Motility, and Cytoskeletal Architecture of Breast Cancer Cells Depend on Keratin 19 and Substrate.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2020Article
- Evidence of Skin Barrier Damage by Cyclic Siloxanes (Silicones)-Using Digital Holographic Microscopy.International journal of molecular sciences · 2020Article
- The photocytotoxicity effect of cationic sulfonated corrole towards lung cancer cells: in vitro and in vivo study.Lasers in medical science · 2019Article
- Whole-Cell Multiparameter Assay for Ricin and Abrin Activity-Based Digital Holographic Microscopy.Toxins · 2019Article
- Digital holography and its multidimensional imaging applications: a review.Microscopy (Oxford, England) · 2018Article
- Noninvasive detection of macrophage activation with single-cell resolution through machine learning.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Quantitative assessment of cancer cell morphology and motility using telecentric digital holographic microscopy and machine learning.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2018Article
- A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells.Journal of visualized experiments : JoVE · 2018Article
- High accuracy label-free classification of single-cell kinetic states from holographic cytometry of human melanoma cells.Scientific reports · 2017Article
- Supervised classification of etoposide-treatedJournal of medical imaging (Bellingham, Wash.) · 2017Article
- Label-free fingerprinting of tumor cells in bulk flow using inline digital holographic microscopy.Biomedical optics express · 2017Article
- Influence of salinomycin treatment on division and movement of individual cancer cells cultured in normoxia or hypoxia evaluated with time-lapse digital holographic microscopy.Cell cycle (Georgetown, Tex.) · 2017Article
- Reversal of Chemoresistance in Ovarian Cancer by Co-Delivery of a P-Glycoprotein Inhibitor and Paclitaxel in a Liposomal Platform.Molecular cancer therapeutics · 2016Article
- Improved Tracking and Resolution of Bacteria in Holographic Microscopy Using Dye and Fluorescent Protein Labeling.Frontiers in chemistry · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Digital holographic microscopy (DHM) has emerged as a powerful non-invasive tool for cell analysis. It has the capacity to analyse multiple parameters simultaneously, such as cell- number, confluence and phase volume. This is done while cells are still adhered and growing in their culture flask. The aim of this study was to investigate whether DHM was able to monitor drug-induced cell cycle arrest in cultured cells and thus provide a non-disruptive alternative to flow cytometry. DHM parameters from G1 and G2/M cell cycle arrested L929 mouse fibroblast cells were collected. Cell cycle arrest was verified with flow cytometry. This study shows that DHM is able to monitor phase volume changes corresponding to either a G1 or G2/M cell cycle arrest. G1-phase arrest with staurosporine correlated with a decrease in the average cell phase volume and G2/M-phase arrest with colcemid and etoposide correlated with an increase in the average cell phase volume. Importantly, DHM analysis of average cell phase volume was of comparable accuracy to flow cytometric measurement of cell cycle phase distribution as recorded following dose-dependent treatment with etoposide. Average cell phase volume changes in response to treatment with cell cycle arresting compounds could therefore be used as a DHM marker for monitoring cell cycle arrest in cultured mammalian cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.