ArticlePloS one2014
Development and evaluation of a panel of filovirus sequence capture probes for pathogen detection by next-generation sequencing.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- Bundibugyo virus disease evidence map: a rapid research needs appraisal.EClinicalMedicine · 2026Article
- Methods, applications, and computational challenges in bait capture enrichment.Cell reports methods · 2025Review
- [Confirmatory Diagnosis of Re-Emerging Filoviruses].Jugan geon-gang gwa jilbyeong · 2023Review
- Highly multiplexed targeted sequencing strategy for infectious disease surveillance.BMC biotechnology · 2023Article
- Diagnostics of Ebola virus.Frontiers in public health · 2023Review
- Article
- Metagenomic sequencing with spiked primer enrichment for viral diagnostics and genomic surveillance.Nature microbiology · 2020Article
- FDA-ARGOS is a database with public quality-controlled reference genomes for diagnostic use and regulatory science.Nature communications · 2019Article
- A highly multiplexed broad pathogen detection assay for infectious disease diagnostics.PLoS neglected tropical diseases · 2018Article
- Genomic Epidemiology Reconstructs the Introduction and Spread of Zika Virus in Central America and Mexico.Cell host & microbe · 2018Article
- The current landscape of nucleic acid tests for filovirus detection.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2018Review
- Targeted Sequencing of Respiratory Viruses in Clinical Specimens for Pathogen Identification and Genome-Wide Analysis.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Targeted Enrichment for Pathogen Detection and Characterization in Three Felid Species.Journal of clinical microbiology · 2017Article
- Neglected filoviruses.FEMS microbiology reviews · 2016Review
- Targeted next-generation sequencing for the detection of ciprofloxacin resistance markers using molecular inversion probes.Scientific reports · 2016Article
- Enhanced virome sequencing using targeted sequence capture.Genome research · 2015Article
- Evaluation of Signature Erosion in Ebola Virus Due to Genomic Drift and Its Impact on the Performance of Diagnostic Assays.Viruses · 2015Article
- Insights from 20 years of bacterial genome sequencing.Functional & integrative genomics · 2015Review
- Pre-symptomatic diagnosis and treatment of filovirus diseases.Frontiers in microbiology · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A detailed understanding of the circulating pathogens in a particular geographic location aids in effectively utilizing targeted, rapid diagnostic assays, thus allowing for appropriate therapeutic and containment procedures. This is especially important in regions prevalent for highly pathogenic viruses co-circulating with other endemic pathogens such as the malaria parasite. The importance of biosurveillance is highlighted by the ongoing Ebola virus disease outbreak in West Africa. For example, a more comprehensive assessment of the regional pathogens could have identified the risk of a filovirus disease outbreak earlier and led to an improved diagnostic and response capacity in the region. In this context, being able to rapidly screen a single sample for multiple pathogens in a single tube reaction could improve both diagnostics as well as pathogen surveillance. Here, probes were designed to capture identifying filovirus sequence for the ebolaviruses Sudan, Ebola, Reston, Taï Forest, and Bundibugyo and the Marburg virus variants Musoke, Ci67, and Angola. These probes were combined into a single probe panel, and the captured filovirus sequence was successfully identified using the MiSeq next-generation sequencing platform. This panel was then used to identify the specific filovirus from nonhuman primates experimentally infected with Ebola virus as well as Bundibugyo virus in human sera samples from the Democratic Republic of the Congo, thus demonstrating the utility for pathogen detection using clinical samples. While not as sensitive and rapid as real-time PCR, this panel, along with incorporating additional sequence capture probe panels, could be used for broad pathogen screening and biosurveillance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.