Evidence map›Paper›PMID 25206900›Full record

ArticleNeural regeneration research2014

Fusion protein of single-chain variable domain fragments for treatment of myasthenia gravis.

Fangfang Li, Fanping Meng, Quanxin Jin, Changyuan Sun, Yingxin Li, Honghua Li, Songzhu Jin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neural regeneration research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Fangfang LiDepartment of Immunology and Pathogenic Biology, College of Medicine, Yanbian University, Yanji, Jilin Province, China.
Fanping MengDepartment of Immunology and Pathogenic Biology, College of Medicine, Yanbian University, Yanji, Jilin Province, China.
Quanxin JinDepartment of Immunology and Pathogenic Biology, College of Medicine, Yanbian University, Yanji, Jilin Province, China.
Changyuan SunDepartment of Immunology and Pathogenic Biology, College of Medicine, Yanbian University, Yanji, Jilin Province, China.
Yingxin LiDepartment of Immunology and Pathogenic Biology, College of Medicine, Yanbian University, Yanji, Jilin Province, China.
Honghua LiDepartment of Immunology and Pathogenic Biology, College of Medicine, Yanbian University, Yanji, Jilin Province, China.
Songzhu JinDepartment of Immunology and Pathogenic Biology, College of Medicine, Yanbian University, Yanji, Jilin Province, China.
Yanbian University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single-chain variable domain fragment (scFv) 637 is an antigen-specific scFv of myasthenia gravis. In this study, scFv and human serum albumin genes were conjugated and the fusion protein was expressed in Pichia pastoris. The affinity of scFv-human serum albumin fusion protein to bind to acetylcholine receptor at the neuromuscular junction of human intercostal muscles was detected by immunofluorescence staining. The ability of the fusion protein to block myasthenia gravis patient sera binding to acetylcholine receptors and its stability in healthy serum were measured by competitive ELISA. The results showed that the inhibition rate was 2.0-77.4%, and the stability of fusion protein in static healthy sera was about 3 days. This approach suggests the scFv-human serum albumin is a potential candidate for specific immunosuppressive therapy of myasthenia gravis.

Indexed as

acetylcholine receptoranti-acetylcholine receptor antibodyautoimmune diseasefusion proteinhuman serum albuminimmunosuppressive therapymyasthenia gravisnerve regenerationneural regenerationNSFC grantsingle-chain variable domain fragment

Identifiers

PMID25206900
PMCPMC4146252
OpenAlexW1984905884

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.