ArticleNeural regeneration research2013
Growth and differentiation of neural stem cells in a three-dimensional collagen gel scaffold.
Article in Neural regeneration research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Applications of synthetic biology in biomedicine.Molecular biomedicine · 2026Review
- Dynamic Interplay Between miR-124-3p and EGF in the Regulation of Overgrowth via RNA Signaling.Biomolecules · 2025Article
- Effects of amyloid-β-mimicking peptide hydrogel matrix on neuronal progenitor cell phenotype.Acta biomaterialia · 2024Article
- Neurogenic Cell Behavior in 3D Culture Enhanced Within a Highly Compliant Synthetic Hydrogel Platform Formed via Competitive Crosslinking.Cellular and molecular bioengineering · 2024Article
- Stem cell-based approaches for developmental neurotoxicity testing.Frontiers in toxicology · 2024Review
- Enhanced growth and differentiation of neural stem cells on alginate/collagen/reduced graphene oxide composite hydrogel incorporated with lithium chloride.BioImpacts : BI · 2023Article
- Application and prospects of high-throughput screening forWorld journal of stem cells · 2022Review
- Conductive Collagen-Based Hydrogel Combined With Electrical Stimulation to Promote Neural Stem Cell Proliferation and Differentiation.Frontiers in bioengineering and biotechnology · 2022Article
- Recombinant collagen scaffolds as substrates for human neural stem/progenitor cells.Journal of biomedical materials research. Part A · 2018Article
- Neural stem cell encapsulation and differentiation in strain promoted crosslinked polyethylene glycol-based hydrogels.Journal of biomaterials applications · 2018Article
- Advances in Nano Neuroscience: From Nanomaterials to Nanotools.Frontiers in neuroscience · 2018Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Collagen protein is an ideal scaffold material for the transplantation of neural stem cells. In this study, rat neural stem cells were seeded into a three-dimensional collagen gel scaffold, with suspension cultured neural stem cells being used as a control group. Neural stem cells, which were cultured in medium containing epidermal growth factor and basic fibroblast growth factor, actively expanded and formed neurospheres in both culture groups. In serum-free medium conditions, the processes extended from neurospheres in the collagen gel group were much longer than those in the suspension culture group. Immunofluorescence staining showed that neurospheres cultured in collagen gels were stained positive for nestin and differentiated cells were stained positive for the neuronal marker βIII-tubulin, the astrocytic marker glial fibrillary acidic protein and the oligodendrocytic marker 2',3'-cyclic nucleotide 3'-phosphodiesterase. Compared with neurospheres cultured in suspension, the differentiation potential of neural stem cells cultured in collagen gels increased, with the formation of neurons at an early stage. Our results show that the three-dimensional collagen gel culture system is superior to suspension culture in the proliferation, differentiation and process outgrowth of neural stem cells.
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