Evidence map›Paper›PMID 25206596›Full record

ArticleNeural regeneration research2013

Cloning and bioinformatical analysis of the N-terminus of the sonic hedgehog gene.

Yi Zhang, Shu Zhao, Weiren Dong, Suifen He, Haihong Wang, Lihua Zhang, Yinjuan Tang, Jiasong Guo, Suiqun Guo

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neural regeneration research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 98% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Yi ZhangSchool of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, Guangdong Province, China ; Department of Gynecology and Obstetrics, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, Guangdong Province, China.
Shu ZhaoSchool of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
Weiren DongSchool of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
Suifen HeDepartment of Laboratory, Shunde Guizhou Hospital, Foshan 528305, Guangdong Province, China.
Haihong WangSchool of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
Lihua ZhangSchool of Medical Sciences, Foshan University, Foshan 528000, Guangdong Province, China.
Yinjuan TangDepartment of Histology & Embryology, Xiangnan College, Chenzhou 418000, Hunan Province, China.
Jiasong GuoDepartment of Gynecology and Obstetrics, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, Guangdong Province, China.
Suiqun GuoDepartment of Gynecology and Obstetrics, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, Guangdong Province, China.
Southern Medical University · CNThird Affiliated Hospital of Southern Medical University · CNFoshan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The sonic hedgehog protein not only plays a key role in early embryonic development, but also has essential effects on the adult nervous system, including neural stem cell proliferation, differentiation, migration and neuronal axon guidance. The N-terminal fragment of sonic hedgehog is the key functional element in this process. Therefore, this study aimed to clone and analyze the N-terminal fragment of the sonic hedgehog gene. Total RNA was extracted from the notochord of a Sprague-Dawley rat at embryonic day 9 and the N-terminal fragment of sonic hedgehog was amplified by nested reverse transcription-PCR. The N-terminal fragment of the sonic hedgehog gene was successfully cloned. The secondary and tertiary structures of the N-terminal fragment of the sonic hedgehog protein were predicted using Jpred and Phyre online.

Indexed as

basic researchcentral nervous systemcloningdevelopmental neurobiologygrants-supported papernested reverse transcription-PCRneural regenerationneuroregenerationphotographs-containing paperratssecondary structuresonic hedgehog proteintertiary structure

Identifiers

PMID25206596
PMCPMC4107527
OpenAlexW309867407

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.