Evidence map›Paper›PMID 25205844›Full record

ArticleStem cells translational medicine2014

Microfluidic Isolation of CD34-Positive Skin Cells Enables Regeneration of Hair and Sebaceous Glands In Vivo.

Beili Zhu, Yaakov Nahmias, Martin L Yarmush, Shashi K Murthy

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells translational medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 14 citations in OpenAlex.

  1. Review
  2. [Analysis of the types and functions of CD34Zhonghua shao shang yu chuang mian xiu fu za zhi · 2024
    Article
  3. Review
  4. Article
  5. Article
  6. Article
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  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Beili ZhuDepartment of Chemical Engineering and Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, Massachusetts, USA; Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospital for Children, Boston, Massachusetts, USA; Grass Center for Bioengineering, The Hebrew University of Jerusalem, Jerusalem, Israel; Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey, USA.
Yaakov NahmiasDepartment of Chemical Engineering and Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, Massachusetts, USA; Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospital for Children, Boston, Massachusetts, USA; Grass Center for Bioengineering, The Hebrew University of Jerusalem, Jerusalem, Israel; Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey, USA.
Martin L YarmushDepartment of Chemical Engineering and Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, Massachusetts, USA; Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospital for Children, Boston, Massachusetts, USA; Grass Center for Bioengineering, The Hebrew University of Jerusalem, Jerusalem, Israel; Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey, USA.
Shashi K MurthyDepartment of Chemical Engineering and Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, Massachusetts, USA; Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospital for Children, Boston, Massachusetts, USA; Grass Center for Bioengineering, The Hebrew University of Jerusalem, Jerusalem, Israel; Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey, USA s.murthy@neu.edu.
Northeastern University · USMassachusetts General Hospital · USShriners Hospitals for Children - Erie · US

Funding

Bio-MicroElectroMechanical Systems Resource CenterP41EB002503 · NIBIB · MASSACHUSETTS GENERAL HOSPITAL · PI TONER, MEHMET · 2004 to 2018
$17.4M
Microfluidic Cell Separation for Tissue Engineering and Regenerative MedicineR01EB009327 · NIBIB · NORTHEASTERN UNIVERSITY · PI MURTHY, SHASHI · 2010 to 2012
$1.8M
NIBIB NIH HHS P41 EB002503NIBIB NIH HHS R01 EB009327NIBIB NIH HHS R01-EB009327
6 · The paper itself

Abstract

Skin stem cells resident in the bulge area of hair follicles and at the basal layer of the epidermis are multipotent and able to self-renew when transplanted into full-thickness defects in nude mice. Based on cell surface markers such as CD34 and the α6-integrin, skin stem cells can be extracted from tissue-derived cell suspensions for engraftment using the gold standard cell separation technique of fluorescence-activated cell sorting (FACS). This paper describes an alternative separation method using microfluidic devices coated with degradable antibody-functionalized hydrogels. The microfluidic method allows direct injection of tissue digestate (no preprocessing tagging of cells is needed), is fast (45 minutes from injected sample to purified cells), and scalable. This method is used in this study to isolate CD34-positive (CD34+) cells from murine skin tissue digestate, and the functional capability of these cells is demonstrated by transplantation into nude mice using protocols developed by other groups for FACS-sorted cells. Specifically, the transplantation of microfluidic isolated CD34+ cells along with dermal and epidermal cells was observed to generate significant levels of hair follicles and sebaceous glands consistent with those observed previously with FACS-sorted cells.

Indexed as

RegenerationStem Cell TransplantationAllograftsAnimalsAntigens, CD34Cell SeparationHairIntegrin alpha6MaleMiceMice, Inbred BALB CMice, NudeMicrofluidic Analytical TechniquesSebaceous GlandsStem CellsAntigens, CD34Integrin alpha6CD34-positive stem cellsMicrofluidic devicesNude miceSkin graft site

Identifiers

PMID25205844
PMCPMC4214846
OpenAlexW2138075749

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.