ArticleProceedings of the National Academy of Sciences of the United States of America2014
Invariant natural killer T cells act as an extravascular cytotoxic barrier for joint-invading Lyme Borrelia.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed, 67 citations in OpenAlex.
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- Characterizing the impact of intracutaneous dissemination on host responses duringFrontiers in immunology · 2026Article
- Extravasation of Borrelia burgdorferi Across the Blood-Brain Barrier is an Extremely Rare Event.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Bridging the gap: Insights in the immunopathology of Lyme borreliosis.European journal of immunology · 2024Review
- invariant Natural Killer T Cells Modulate the Peritoneal Macrophage Response to Polymicrobial Sepsis.The Journal of surgical research · 2024Article
- Choreography of Lyme Disease Spirochete Adhesins To Promote Vascular Escape.Microbiology spectrum · 2023Article
- Cell-Mediated Cytotoxicity in Lyme Arthritis.Arthritis & rheumatology (Hoboken, N.J.) · 2023Article
- Visualization of the Dynamics of Invasion and Intravasation of the Bacterium That Causes Lyme Disease in a Tissue Engineered Dermal Microvessel Model.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Role of NK-Like CD8Infection and immunity · 2022Article
- Differential location of NKT and MAIT cells within lymphoid tissue.Scientific reports · 2022Article
- Methods to Evaluate Bacterial Motility and Its Role in Bacterial-Host Interactions.Microorganisms · 2022Review
- Natural killer cells kill extracellular Pseudomonas aeruginosa using contact-dependent release of granzymes B and H.PLoS pathogens · 2022Article
- Live Imaging.Current issues in molecular biology · 2021Review
- FTY720 Inhibits the Development of Collagen-Induced Arthritis in Mice by Suppressing the Recruitment of CD4Drug design, development and therapy · 2021Article
- Actually Seeing What Is Going on - Intravital Microscopy in Tissue Engineering.Frontiers in bioengineering and biotechnology · 2021Review
- Intravital imaging of interactions between iNKT and kupffer cells to clear free lipids during steatohepatitis.Theranostics · 2021Article
- Lyme Disease Pathogenesis.Current issues in molecular biology · 2021Review
- On invariant T cells and measles: A theory of "innate immune amnesia".PLoS pathogens · 2020Article
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11 authors at 5 institutions in 4 countries.
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Abstract
CXCR6-GFP(+) cells, which encompass 70% invariant natural killer T cells (iNKT cells), have been found primarily patrolling inside blood vessels in the liver. Although the iNKT cells fail to interact with live pathogens, they do respond to bacterial glycolipids presented by CD1d on liver macrophage that have caught the microbe. In contrast, in this study using dual laser multichannel spinning-disk intravital microscopy of joints, the CXCR6-GFP, which also made up 60-70% iNKT cells, were not found in the vasculature but rather closely apposed to and surrounding the outside of blood vessels, and to a lesser extent throughout the extravascular space. These iNKT cells also differed in behavior, responding rapidly and directly to joint-homing pathogens like Borrelia burgdorferi, which causes Lyme disease. These iNKT cells interacted with B. burgdorferi at the vessel wall and disrupted dissemination attempts by these microbes into joints. Successful penetrance of B. burgdorferi out of the vasculature and into the joint tissue was met by a lethal attack by extravascular iNKT cells through a granzyme-dependent pathway, an observation also made in vitro for iNKT cells from joint but not liver or spleen. These results suggest a novel, critical extravascular iNKT cell immune surveillance in joints that functions as a cytotoxic barrier and explains a large increase in pathogen burden of B. burgdorferi in the joint of iNKT cell-deficient mice, and perhaps the greater susceptibility of humans to this pathogen because of fewer iNKT cells in human joints.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.