ArticleCurrent protocols in chemical biology2014
Glycomic analysis of glycans released from glycoproteins using chemical immobilization and mass spectrometry.
Article in Current protocols in chemical biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- A Linkage-specific Sialic Acid Labeling Strategy Reveals Different Site-specific Glycosylation Patterns in SARS-CoV-2 Spike Protein Produced in CHO and HEK Cell Substrates.Frontiers in chemistry · 2021Article
- Glycoengineering of Mammalian Expression Systems on a Cellular Level.Advances in biochemical engineering/biotechnology · 2021Article
- A Pragmatic Guide to Enrichment Strategies for Mass Spectrometry-Based Glycoproteomics.Molecular & cellular proteomics : MCP · 2021Review
- Host-glycan metabolism is regulated by a species-conserved two-component system in Streptococcus pneumoniae.PLoS pathogens · 2020Article
- Reanalysis of Global Proteomic and Phosphoproteomic Data Identified a Large Number of Glycopeptides.Analytical chemistry · 2018Article
- Analytical Scheme Leading to Integrated High-Sensitivity Profiling of Glycosphingolipids Together with N- and O-Glycans from One Sample.Journal of the American Society for Mass Spectrometry · 2018Article
- Identification of Sialic Acid Linkages on Intact Glycopeptides via Differential Chemical Modification Using IntactGIG-HILIC.Journal of the American Society for Mass Spectrometry · 2018Article
- Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans.Journal of analytical methods in chemistry · 2018Article
- N-glycan in cockroach allergen regulates human basophil function.Immunity, inflammation and disease · 2017Article
- High-throughput analysis of N-glycans using AutoTip via glycoprotein immobilization.Scientific reports · 2017Article
- Modification of Sialic Acids on Solid Phase: Accurate Characterization of Protein Sialylation.Analytical chemistry · 2017Article
- Simultaneous quantification of N- and O-glycans using a solid-phase method.Nature protocols · 2017Article
- Simultaneous analyses of N-linked and O-linked glycans of ovarian cancer cells using solid-phase chemoenzymatic method.Clinical proteomics · 2017Article
- Review
- Integrated glycoprotein immobilization method for glycopeptide and glycan analysis of cardiac hypertrophy.Analytical chemistry · 2015Article
- Microfluidic Chip-LC/MS-based Glycomic Analysis Revealed Distinct N-glycan Profile of Rat Serum.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Protein glycosylation is one of most common protein modifications and is involved in many biological activities. N-linked and O-linked glycosylation not only represent abundant glycan modifications, but also are structurally diverse. Mass spectrometry has emerged as a major method for glycomic analysis. However, glycan extraction from proteins and glycan modification are two critical steps in glycomic analysis of glycans using mass spectrometry. In this protocol, we describe a novel and high-throughput method for isolation and modification of glycans from glycoproteins using a chemoenzymatic approach on solid-phase. Proteins are first immobilized to a solid support and unconjugated molecules are washed away; glycans, while still linked to glycoproteins on the solid support, can be treated enzymatically or chemically on solid phase for glycan derivatization. Glycans are then released from the solid support for analysis by mass spectrometry. The procedures outlined are robust and useful for high-throughput glycomic analysis from complex biological or clinical samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.