ArticleLab on a chip2014
Microfluidic device for stem cell differentiation and localized electroporation of postmitotic neurons.
Article in Lab on a chip, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Well Plate-Based Localized Electroporation Workflow for Rapid Optimization of Intracellular Delivery.Bio-protocol · 2024Article
- Mechanism study on the influences of buffer osmotic pressure on microfluidic chip-based cell electrofusion.APL bioengineering · 2024Article
- High-throughput mechanical phenotyping and transcriptomics of single cells.Nature communications · 2024Article
- Single cell transcriptomics reveals reduced stress response in stem cells manipulated using localized electric fields.Materials today. Bio · 2023Article
- Multiplexed high-throughput localized electroporation workflow with deep learning-based analysis for cell engineering.Science advances · 2022Article
- Recent Advances in Microscale Electroporation.Chemical reviews · 2022Review
- Microfluidic platforms for single neuron analysis.Materials today. Bio · 2022Review
- High-Efficient and Dosage-Controllable Intracellular Cargo Delivery through Electrochemical Metal-Organic Hybrid Nanogates.Small science · 2021Article
- In situ electroporation of mammalian cells through SiOScientific reports · 2021Article
- Microfluidics as efficient technology for the isolation and characterization of stem cells.EXCLI journal · 2021Review
- High Throughput and Highly Controllable Methods for In Vitro Intracellular Delivery.Small (Weinheim an der Bergstrasse, Germany) · 2020Review
- Nanofountain Probe Electroporation Enables Versatile Single-Cell Intracellular Delivery and Investigation of Postpulse Electropore Dynamics.Small (Weinheim an der Bergstrasse, Germany) · 2020Article
- Temporal Sampling of Enzymes from Live Cells by Localized Electroporation and Quantification of Activity by SAMDI Mass Spectrometry.Small (Weinheim an der Bergstrasse, Germany) · 2020Article
- Drug Toxicity Evaluation Based on Organ-on-a-chip Technology: A Review.Micromachines · 2020Review
- A Disposable Passive Microfluidic Device for Cell Culturing.Biosensors · 2020Article
- Microfluidic Device for Localized Electroporation.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Reply to Nathamgari et al.: Nanopore electroporation for intracellular delivery of biological macromolecules.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Localized electroporation with track-etched membranes.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Nontoxic nanopore electroporation for effective intracellular delivery of biological macromolecules.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Combined Numerical and Experimental Investigation of Localized Electroporation-Based Cell Transfection and Sampling.ACS nano · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
New techniques to deliver nucleic acids and other molecules for gene editing and gene expression profiling, which can be performed with minimal perturbation to cell growth or differentiation, are essential for advancing biological research. Studying cells in their natural state, with temporal control, is particularly important for primary cells that are derived by differentiation from stem cells and are adherent, e.g., neurons. Existing high-throughput transfection methods either require cells to be in suspension or are highly toxic and limited to a single transfection per experiment. Here we present a microfluidic device that couples on-chip culture of adherent cells and transfection by localized electroporation. Integrated microchannels allow long-term cell culture on the device and repeated temporal transfection. The microfluidic device was validated by first performing electroporation of HeLa and HT1080 cells, with transfection efficiencies of ~95% for propidium iodide and up to 50% for plasmids. Application to primary cells was demonstrated by on-chip differentiation of neural stem cells and transfection of postmitotic neurons with a green fluorescent protein plasmid.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.