ArticleAnnals of biomedical engineering2014
Correlated parameter fit of arrhenius model for thermal denaturation of proteins and cells.
Article in Annals of biomedical engineering, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 82 citations in OpenAlex.
- The kinetic basis of photosynthetic heat tolerance.Nature ecology & evolution · 2026Article
- Activation parameters, enthalpy-entropy compensation and the temperature-dependent activity of enzymes.Protein science : a publication of the Protein Society · 2026Article
- The Nature of Non-Arrhenius Kinetics in the Heat Denaturation of Proteins.International journal of molecular sciences · 2026Article
- Experimental Study on the Process and Performance of Laser Repair of Esophageal Tissue Adding ICG and CNT.Journal of biophotonics · 2026Article
- Strategic selection of multi-parametric laser settings and clinical endpoints in dermatology: an engineering-to-clinical review.Lasers in medical science · 2026Review
- Automated laser retraction for targeted glioblastoma coverage during laser interstitial thermal therapy.Medical physics · 2026Article
- Mathematical Descriptions of Damage to Cells and Tissues Following Heating.Advances in experimental medicine and biology · 2026Article
- Therapeutic Window of Laser Dosimetry for the Treatment of Knee Osteoarthritis.Lasers in surgery and medicine · 2025Article
- Activation parameters, enthalpy-entropy compensation and the temperature-dependent activity of enzymes.bioRxiv : the preprint server for biology · 2025Article
- Higher-order thermal modeling and computational analysis of laser ablation in anisotropic cardiac tissue.Biomechanics and modeling in mechanobiology · 2025Article
- Temperature-Dependent Cytokine Neutralization Induced by Magnetoelectric Nanoparticles: An In Silico Study.International journal of molecular sciences · 2024Article
- Optical signatures of thermal damage on ex-vivo brain, lung and heart tissues using time-domain diffuse optical spectroscopy.Biomedical optics express · 2024Article
- The Nature of the Enthalpy-Entropy Compensation and "Exotic" Arrhenius Parameters in the Denaturation Kinetics of Proteins.International journal of molecular sciences · 2023Article
- Multilayer In Vitro Human Skin Tissue Platforms for Quantitative Burn Injury Investigation.Bioengineering (Basel, Switzerland) · 2023Article
- Highly Accurate and Robust Absolute Quantification of Target Proteins in Formalin-Fixed Paraffin-Embedded (FFPE) Tissues by LC-MS.Analytical chemistry · 2023Article
- Chelation of Zinc with Biogenic Amino Acids: Description of Properties Using Balaban Index, Assessment of Biological Activity onPharmaceuticals (Basel, Switzerland) · 2022Article
- Evaluation of ZnMolecules (Basel, Switzerland) · 2022Article
- Determination of the denaturation temperature of the Spike protein S1 of SARS-CoV-2 (2019 nCoV) by Raman spectroscopy.Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy · 2022Article
- A novel disinfected Trombe wall for space heating and virus inactivation: Concept and performance investigation.Applied energy · 2021Article
- Computational Investigation of Protein Photoinactivation by Molecular Hyperthermia.Journal of biomechanical engineering · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Thermal denaturation of proteins is critical to cell injury, food science and other biomaterial processing. For example protein denaturation correlates strongly with cell death by heating, and is increasingly of interest in focal thermal therapies of cancer and other diseases at temperatures which often exceed 50 °C. The Arrhenius model is a simple yet widely used model for both protein denaturation and cell injury. To establish the utility of the Arrhenius model for protein denaturation at 50 °C and above its sensitivities to the kinetic parameters (activation energy E a and frequency factor A) were carefully examined. We propose a simplified correlated parameter fit to the Arrhenius model by treating E a, as an independent fitting parameter and allowing A to follow dependently. The utility of the correlated parameter fit is demonstrated on thermal denaturation of proteins and cells from the literature as a validation, and new experimental measurements in our lab using FTIR spectroscopy to demonstrate broad applicability of this method. Finally, we demonstrate that the end-temperature within which the denaturation is measured is important and changes the kinetics. Specifically, higher E a and A parameters were found at low end-temperature (50 °C) and reduce as end-temperatures increase to 70 °C. This trend is consistent with Arrhenius parameters for cell injury in the literature that are significantly higher for clonogenics (45-50 °C) vs. membrane dye assays (60-70 °C). Future opportunities to monitor cell injury by spectroscopic measurement of protein denaturation are discussed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.