ArticleJournal of the American Society for Mass Spectrometry2014
High pressure super-heated electrospray ionization mass spectrometry for sub-critical aqueous solution.
Article in Journal of the American Society for Mass Spectrometry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 22 citations in OpenAlex.
- Easy, Robust, and Repeatable Online Acid Cleavage of Proteins in Mobile Phase for Fast Quantitative LC-MS Bottom-Up Protein Analysis─Application for Ricin Detection.Analytical chemistry · 2023Article
- Temperature-Controlled Electrospray Ionization: Recent Progress and Applications.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Review
- Fast protein analysis enabled by high-temperature hydrolysis.Chemical science · 2020Article
- High-Temperature Liquid Chromatography and the Hyphenation with Mass Spectrometry Using High-Pressure Electrospray Ionization.Mass spectrometry (Tokyo, Japan) · 2019Article
- CID fragmentation, H/D exchange and supermetallization of Barnase-Barstar complex.Scientific reports · 2017Article
- Rapid Online Non-Enzymatic Protein Digestion Analysis with High Pressure Superheated ESI-MS.Journal of the American Society for Mass Spectrometry · 2015Article
- When API Mass Spectrometry Meets Super Atmospheric Pressure Ion Sources.Mass spectrometry (Tokyo, Japan) · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The heating of electrospray ion source under atmospheric pressure is limited to the normal boiling point of the solution. The boiling takes place when the vapor pressure of the liquid at a given temperature equals the ambient pressure. By using a high pressure ESI source, which has been developed previously in our laboratory, a stable electrospray ionization of super-heated aqueous solution is performed up to a solution temperature of 180°C. The ion source is pressurized with pure nitrogen to a maximum pressure of 11 atm, and it is coupled to a commercial mass spectrometer via a custom-made ion transport capillary. A booster pump with variable pumping speed is added to the pumping system to regulate the pressure in the first pumping stage at 1 ~ 1.3 Torr. High pressure mass spectrometry is performed on several peptides and proteins to demonstrate its application in the temperature-controlled thermally induced denaturation and dissociation.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.