ArticleTransgenic research2015
Genome edited sheep and cattle.
Article in Transgenic research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
101 citing papers in PubMed, 1 synthesis or guideline pooled it, 272 citations in OpenAlex.
- The ethics of clinical applications of germline genome modification: a systematic review of reasons.Human reproduction (Oxford, England) · 2018Pooled it
- Safe and Stable Germline Transmission of MSTN Mutations in Cattle.Animal genetics · 2026Article
- Non-Coding Negative Regulatory Features in Livestock Genomes: Functional Annotation and Causal Validation.Animals : an open access journal from MDPI · 2026Review
- Generation of germline-transmitting transgenic sheep by piggyBac-mediated transgenesis using pronuclear and cytoplasmic gene injection approaches.Transgenic research · 2026Article
- CRISPR Technology for Livestock Improvement: Advances and Future Directions.Molecular biotechnology · 2026Review
- Production of gene-edited cloned cattle embryos using the CRISPR/EOCas12i system.Frontiers in genome editing · 2026Article
- Comprehensive analysis ofAnimal biotechnology · 2025Article
- MSTN gene editing does not alter reproductive capacity but modifies gestational metabolism in Mongolian cattle.BMC veterinary research · 2025Article
- Cytosine base editor-mediated high-efficiency myostatin editing in Hu sheep.Functional & integrative genomics · 2025Article
- New developments in biotechnology applied to animals: An assessment of the adequacy and sufficiency of current EFSA guidance for animal risk assessment.EFSA journal. European Food Safety Authority · 2025Article
- Impacts of heat stress on growth performance and its mitigation in small ruminants.Animal frontiers : the review magazine of animal agriculture · 2025Article
- Agricultural biotechnology in China: product development, commercialization, and perspectives.aBIOTECH · 2025Review
- Genetic advancements and future directions in ruminant livestock breeding: from reference genomes to multiomics innovations.Science China. Life sciences · 2025Review
- Advancements in Plant Gene Editing Technology: From Construct Design to Enhanced Transformation Efficiency.Biotechnology journal · 2024Review
- Commercial perspectives: Genome editing as a breeding tool for health and well-being in dairy cattle.JDS communications · 2024Article
- An overview of recent technological developments in bovine genomics.Veterinary and animal science · 2024Review
- Base Characteristics, Preservation Methods, and Assessment of the Genetic Diversity of Autochthonous Breeds of Cattle, Sheep and Pigs in Serbia: A Review.Animals : an open access journal from MDPI · 2024Review
- Current status and future of gene engineering in livestock.BMB reports · 2024Review
- Gene editing in livestock: innovations and applications.Animal reproduction · 2024Article
- Outlook on genome editing application to cattle.Journal of veterinary science · 2024Review
41 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
Abstract
Genome editing tools enable efficient and accurate genome manipulation. An enhanced ability to modify the genomes of livestock species could be utilized to improve disease resistance, productivity or breeding capability as well as the generation of new biomedical models. To date, with respect to the direct injection of genome editor mRNA into livestock zygotes, this technology has been limited to the generation of pigs with edited genomes. To capture the far-reaching applications of gene-editing, from disease modelling to agricultural improvement, the technology must be easily applied to a number of species using a variety of approaches. In this study, we demonstrate zygote injection of TALEN mRNA can also produce gene-edited cattle and sheep. In both species we have targeted the myostatin (MSTN) gene. In addition, we report a critical innovation for application of gene-editing to the cattle industry whereby gene-edited calves can be produced with specified genetics by ovum pickup, in vitro fertilization and zygote microinjection (OPU-IVF-ZM). This provides a practical alternative to somatic cell nuclear transfer for gene knockout or introgression of desirable alleles into a target breed/genetic line.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.