ArticleDevelopmental cell2014
An ankyrin repeat domain of AKR2 drives chloroplast targeting through coincident binding of two chloroplast lipids.
Article in Developmental cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 55 citations in OpenAlex.
- Cellular Partners of Tobamoviral Movement Proteins.International journal of molecular sciences · 2025Review
- A distinct dimer configuration of a diatom Get3 forming a tetrameric complex with its tail-anchored membrane cargo.BMC biology · 2024Article
- NPR1 Translocation from Chloroplast to Nucleus Activates Plant Tolerance to Salt Stress.Antioxidants (Basel, Switzerland) · 2023Article
- Understanding protein import in diverse non-green plastids.Frontiers in genetics · 2023Review
- The Ankyrin Repeat Protein RARP-1 Is a Periplasmic Factor That SupportsJournal of bacteriology · 2022Article
- New Insights into the Chloroplast Outer Membrane Proteome and Associated Targeting Pathways.International journal of molecular sciences · 2022Review
- Article
- Protein-protein interactions in plant antioxidant defense.Frontiers in plant science · 2022Review
- Go your own way: membrane-targeting sequences.Plant physiology · 2021Article
- The ankyrin repeat-containing protein MdANK2B regulates salt tolerance and ABA sensitivity in Malus domestica.Plant cell reports · 2021Article
- Genome-wide identification of ubiquitin proteasome subunits as superior reference genes for transcript normalization during receptacle development in strawberry cultivars.BMC genomics · 2021Article
- Genome wide association mapping of epi-cuticular wax genes inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- AKR2A participates in the regulation of cotton fibre development by modulating biosynthesis of very-long-chain fatty acids.Plant biotechnology journal · 2020Article
- Ankyrins in human health and disease - an update of recent experimental findings.Archives of medical science : AMS · 2020Article
- Ankyrin repeat-containing N-Ank proteins shape cellular membranes.Nature cell biology · 2019Article
- Role of Galactolipids in Plastid Differentiation Before and After Light Exposure.Plants (Basel, Switzerland) · 2019Review
- Membrane-Deformation Ability of ANKHD1 Is Involved in the Early Endosome Enlargement.iScience · 2019Article
- Biogenesis of chloroplast outer envelope membrane proteins.Plant cell reports · 2019Review
- Evolution of protein transport to the chloroplast envelope membranes.Photosynthesis research · 2018Review
- TRPA1 ankyrin repeat six interacts with a small molecule inhibitor chemotype.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
Abstract
In organellogenesis of the chloroplast from endosymbiotic cyanobacteria, the establishment of protein-targeting mechanisms to the chloroplast should have been pivotal. However, it is still mysterious how these mechanisms were established and how they work in plant cells. Here we show that AKR2A, the cytosolic targeting factor for chloroplast outer membrane (COM) proteins, evolved from the ankyrin repeat domain (ARD) of the host cell by stepwise extensions of its N-terminal domain and that two lipids, monogalactosyldiacylglycerol (MGDG) and phosphatidylglycerol (PG), of the endosymbiont were selected to function as the AKR2A receptor. Structural analysis, molecular modeling, and mutational analysis of the ARD identified two adjacent sites for coincidental and synergistic binding of MGDG and PG. Based on these findings, we propose that the targeting mechanism of COM proteins was established using components from both the endosymbiont and host cell through a modification of the protein-protein-interacting ARD into a lipid binding domain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.