Evidence map›Paper›PMID 25202828›Full record

ArticleThe Journal of investigative dermatology2015

Fate and plasticity of the epidermis in response to congenital activation of BRAF.

Suguna R Krishnaswami, Shantanu Kumar, Phillip Ordoukhanian, Benjamin D Yu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of investigative dermatology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Suguna R KrishnaswamiDivision of Dermatology, Department of Medicine, Institute for Genomic Medicine, Stem Cell Program, University of California, San Diego, La Jolla, California, USA.
Shantanu KumarDivision of Dermatology, Department of Medicine, Institute for Genomic Medicine, Stem Cell Program, University of California, San Diego, La Jolla, California, USA.
Phillip OrdoukhanianNext Generation Sequencing Core, The Scripps Research Institute, San Diego, California, USA.
Benjamin D YuDivision of Dermatology, Department of Medicine, Institute for Genomic Medicine, Stem Cell Program, University of California, San Diego, La Jolla, California, USA.
University of California, San Diego · USScripps Research Institute · US

Funding

RAS Signaling in Skin Stem Cell Proliferation and FateR01AR056667 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SEN, GEORGE L · 2009 to 2013
$1.5M
NIAMS NIH HHS AR056667NIAMS NIH HHS R01 AR056667
6 · The paper itself

Abstract

Germline and somatic mutations in RAS and its downstream effectors are found in several congenital conditions affecting the skin. Here we demonstrate that activation of BRAF in the embryonic mouse ectoderm triggers both craniofacial and skin defects, including hyperproliferation, loss of spinous and granular keratinocyte differentiation, and cleft palate. RNA sequencing of the epidermis confirmed these findings but unexpectedly revealed evidence of continued epidermal maturation, expression of >80% of epidermal differentiation complex genes, and formation of a hydrophobic barrier. Spinous and granular differentiation were restored by pharmacologic inhibition of MAPK/ERK kinase or BRAF. However, restoration of epidermal differentiation was non-cell autonomous and required dermal tissue to be present in tissue recombination studies. These studies indicate that early activation of the RAF signaling pathway in the ectoderm has effects on specific steps of epidermal differentiation, which may be amenable to treatment with currently available pharmacologic inhibitors.

Indexed as

Cell LineageAnimalsCell DifferentiationEctodermEnzyme ActivationEpidermisFemaleMaleMAP Kinase Signaling SystemMiceMitogen-Activated Protein Kinase KinasesPregnancyProto-Oncogene Proteins B-rafras ProteinsBraf protein, mouseMitogen-Activated Protein Kinase KinasesProto-Oncogene Proteins B-rafras Proteins

Identifiers

PMID25202828
PMCPMC4289449
OpenAlexW1992000756

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.