ArticleApplications in plant sciences2014
3D Plant cell architecture of Arabidopsis thaliana (Brassicaceae) using focused ion beam-scanning electron microscopy.
Article in Applications in plant sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed.
- Structural and Functional Characterization of EXPO-Derived Extracellular Vesicles in Plants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Characterization of Grain Structure Using Micro-CT and Identification of Related Candidate Genes by QTL Mapping in Foxtail Millet.Plants (Basel, Switzerland) · 2025Article
- Cryo-SEM and large volume FIB-SEM of Arabidopsis cotyledons: Degradation of lipid bodies, biogenesis of glyoxysomes and reorganisation of organelles during germination.Journal of microscopy · 2025Article
- Research on the evolutionary history of the morphological structure of cotton seeds: a new perspective based on high-resolution micro-CT technology.Frontiers in plant science · 2023Article
- Volumetric 3D reconstruction of plant leaf cells using SEM, ion milling, TEM, and serial sectioning.Planta · 2022Article
- X-ray microscopy enables multiscale high-resolution 3D imaging of plant cells, tissues, and organs.Plant physiology · 2022Article
- Correlations between lignin content and structural robustness in plants revealed by X-ray ptychography.Scientific reports · 2020Article
- Quantitative imaging of plants: multi-scale data for better plant anatomy.Journal of experimental botany · 2018Article
- Three-dimensional intracellular structure of a whole rice mesophyll cell observed with FIB-SEM.Annals of botany · 2017Article
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3 authors.
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Abstract
unlabelledPREMISE OF THE STUDY: Focused ion beam-scanning electron microscopy (FIB-SEM) combines the ability to sequentially mill the sample surface and obtain SEM images that can be used to create 3D renderings with micron-level resolution. We have applied FIB-SEM to study Arabidopsis cell architecture. The goal was to determine the efficacy of this technique in plant tissue and cellular studies and to demonstrate its usefulness in studying cell and organelle architecture and distribution. •
methodsSeed aleurone, leaf mesophyll, stem cortex, root cortex, and petal lamina from Arabidopsis were fixed and embedded for electron microscopy using protocols developed for animal tissues and modified for use with plant cells. Each sample was sectioned using the FIB and imaged with SEM. These serial images were assembled to produce 3D renderings of each cell type. •
resultsOrganelles such as nuclei and chloroplasts were easily identifiable, and other structures such as endoplasmic reticula, lipid bodies, and starch grains were distinguishable in each tissue. • DISCUSSION: The application of FIB-SEM produced 3D renderings of five plant cell types and offered unique views of their shapes and internal content. These results demonstrate the usefulness of FIB-SEM for organelle distribution and cell architecture studies.
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