Evidence map›Paper›PMID 25202623›Full record

ArticleApplications in plant sciences2014

Characterization of microsatellite loci in the Himalayan lichen fungus Lobaria pindarensis (Lobariaceae).

Shiva Devkota, Carolina Cornejo, Silke Werth, Ram Prasad Chaudhary, Christoph Scheidegger

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Article in Applications in plant sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

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5citing papers in PubMed
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5 citing papers in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Shiva DevkotaSwiss Federal Research Institute WSL, Zürcherstrasse 111, CH-8903 Birmensdorf, Switzerland ; Central Department of Botany, Tribhuvan University, Kirtipur, Nepal.
Carolina CornejoSwiss Federal Research Institute WSL, Zürcherstrasse 111, CH-8903 Birmensdorf, Switzerland.
Silke WerthSwiss Federal Research Institute WSL, Zürcherstrasse 111, CH-8903 Birmensdorf, Switzerland ; Faculty of Life and Environmental Sciences, University of Iceland, Reykjavik, Iceland.
Ram Prasad ChaudharyCentral Department of Botany, Tribhuvan University, Kirtipur, Nepal ; Research Centre for Applied Science and Technology (RECAST), Tribhuvan University, Kirtipur, Nepal.
Christoph ScheideggerSwiss Federal Research Institute WSL, Zürcherstrasse 111, CH-8903 Birmensdorf, Switzerland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

unlabelledPREMISE OF THE STUDY: Microsatellite loci were developed for the rare, Himalayan, endemic haploid lichen fungus, Lobaria pindarensis, to study its population subdivision and the species' response to forest disturbance and fragmentation. • METHODS AND

resultsWe developed 18 polymorphic microsatellite markers using 454 pyrosequencing data and assessed them in 109 individuals. The number of alleles per locus ranged from three to 11 with an average of 6.9. Nei's unbiased gene diversity, averaged over loci, ranged from 0.514 to 0.685 in the three populations studied. The cross-amplification success with related species (L. chinensis, L. gyrophorica, L. isidiophora, L. orientalis, L. pulmonaria, L. spathulata, and Lobaria sp.) was generally high and decreased with decreasing relationship to L. pindarensis. •

conclusionsThe new markers will allow the study of genetic diversity and differentiation within L. pindarensis across its distribution. Moreover, they will enable us to study the effects of forest management on the genetic population structure of this tree-colonizing lichen and to carry out population genetic studies of related species in East Asia.

Indexed as

AscomycetesHimalayaslichen-forming fungiLobaria pindarensismicrosatellitespopulation subdivision

Identifiers

PMID25202623
PMCPMC4103109

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