ArticleApplications in plant sciences2014
A long PCR-based approach for DNA enrichment prior to next-generation sequencing for systematic studies.
Article in Applications in plant sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Reconstructing the survival history of relict herbs endemic to the heavy snowfall regions of Japan during the Pleistocene.Journal of plant research · 2026Article
- Repeated loss of plastid NDH during evolution of land plants.Annals of botany · 2026Review
- Exploring Angiosperms353: Developing and applying a universal toolkit for flowering plant phylogenomics.Applications in plant sciences · 2021Article
- Green giant-a tiny chloroplast genome with mighty power to produce high-value proteins: history and phylogeny.Plant biotechnology journal · 2021Review
- Can we use it? On the utility of de novo and reference-based assembly of Nanopore data for plant plastome sequencing.PloS one · 2020Article
- Article
- Primers forApplications in plant sciences · 2017Article
- Strategies for complete plastid genome sequencing.Molecular ecology resources · 2017Review
- Primers forApplications in plant sciences · 2017Article
- Application of a simplified method of chloroplast enrichment to small amounts of tissue for chloroplast genome sequencing.Applications in plant sciences · 2017Article
- Article
- Limited mitogenomic degradation in response to a parasitic lifestyle in Orobanchaceae.Scientific reports · 2016Article
- Mechanistic model of evolutionary rate variation en route to a nonphotosynthetic lifestyle in plants.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Detecting and Characterizing the Highly Divergent Plastid Genome of the Nonphotosynthetic Parasitic Plant Hydnora visseri (Hydnoraceae).Genome biology and evolution · 2016Article
- Complete Plastid Genome of the Recent Holoparasite Lathraea squamaria Reveals Earliest Stages of Plastome Reduction in Orobanchaceae.PloS one · 2016Article
- Article
- Genome and metagenome sequencing: Using the human methyl-binding domain to partition genomic DNA derived from plant tissues.Applications in plant sciences · 2014Article
- A long PCR-based approach for DNA enrichment prior to next-generation sequencing for systematic studies.Applications in plant sciences · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledPREMISE OF THE STUDY: We present an alternative approach for molecular systematic studies that combines long PCR and next-generation sequencing. Our approach can be used to generate templates from any DNA source for next-generation sequencing. Here we test our approach by amplifying complete chloroplast genomes, and we present a set of 58 potentially universal primers for angiosperms to do so. Additionally, this approach is likely to be particularly useful for nuclear and mitochondrial regions. • METHODS AND
resultsChloroplast genomes of 30 species across angiosperms were amplified to test our approach. Amplification success varied depending on whether PCR conditions were optimized for a given taxon. To further test our approach, some amplicons were sequenced on an Illumina HiSeq 2000. •
conclusionsAlthough here we tested this approach by sequencing plastomes, long PCR amplicons could be generated using DNA from any genome, expanding the possibilities of this approach for molecular systematic studies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.