Evidence map›Paper›PMID 25202552›Full record

ArticleApplications in plant sciences2013

Primers for low-copy nuclear genes in the Hawaiian endemic Clermontia (Campanulaceae) and cross-amplification in Lobelioideae.

Yohan Pillon, Jennifer Johansen, Tomoko Sakishima, Srikar Chamala, W Brad Barbazuk, Elizabeth A Stacy

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applications in plant sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Yohan PillonTropical Conservation Biology and Environmental Science Program, University of Hawai'i at Hilo, 200 West Kawili Street, Hilo, Hawaii 96720 USA.
Jennifer JohansenTropical Conservation Biology and Environmental Science Program, University of Hawai'i at Hilo, 200 West Kawili Street, Hilo, Hawaii 96720 USA.
Tomoko SakishimaTropical Conservation Biology and Environmental Science Program, University of Hawai'i at Hilo, 200 West Kawili Street, Hilo, Hawaii 96720 USA.
Srikar ChamalaDepartment of Biology, University of Florida, Gainesville, Florida 32611 USA ; Genetics Institute, University of Florida, Gainesville, Florida 32610 USA.
W Brad BarbazukDepartment of Biology, University of Florida, Gainesville, Florida 32611 USA ; Genetics Institute, University of Florida, Gainesville, Florida 32610 USA.
Elizabeth A StacyTropical Conservation Biology and Environmental Science Program, University of Hawai'i at Hilo, 200 West Kawili Street, Hilo, Hawaii 96720 USA.
University of Hawaii at Hilo · USUniversity of Florida · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

unlabelledPREMISE OF THE STUDY: Primers were developed to amplify 12 intron-less, low-copy nuclear genes in the Hawaiian genus Clermontia (Campanulaceae), a suspected tetraploid. • METHODS AND

resultsData from a pooled 454 titanium run of the partial transcriptomes of seven Clermontia species were used to identify the loci of interest. Most loci were amplified and sequenced directly with success in a representative selection of lobeliads even though several of these loci turned out to be duplicated. Levels of variation were comparable to those observed in commonly used plastid and ribosomal markers. •

conclusionsWe found evidence of a genome duplication that likely predates the diversification of the Hawaiian lobeliads. Some genes nevertheless appear to be single-copy and should be useful for phylogenetic studies of Clermontia or the entire Lobelioideae subfamily.

Indexed as

ClermontiaHawai‘iLobelioideaelow-copy nuclear genesnext-generation sequencingpolyploidy

Identifiers

PMID25202552
PMCPMC4105025
OpenAlexW2106408178

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.