ArticleApplications in plant sciences2013
A method for extracting high-quality RNA from diverse plants for next-generation sequencing and gene expression analyses.
Article in Applications in plant sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed.
- Integrative utilization of genomic resources for improved phylogenetic resolution in Sonerileae (Melastomataceae).American journal of botany · 2026Article
- Optimising RNA extraction for paddy bulk soil samples for metatranscriptome sequencing.BMC research notes · 2025Article
- Effect of fieldwork-friendly coffee blender-based extraction methods and leaf tissue storage on the transcriptome of non-model plants.Journal of plant research · 2025Article
- A robust absolute quantification protocol for potato virus Y based on reverse transcription-quantitative PCR.microPublication biology · 2025Article
- Development of an infiltration-based RNA preservation method for cryogen-free storage of leaves for gene expression analyses in field-grown plants.Plant methods · 2024Article
- Assessing the effect of sample storage time on viral detection using a rapid and cost-effective CTAB-based extraction method.Plant methods · 2024Article
- An Optimized and Cost-Effective RNA Extraction Method for Secondary Metabolite-Enriched Tissues of Norway Spruce (Plants (Basel, Switzerland) · 2024Article
- Suppression of tomato wilt by cell-free supernatants of Acinetobacter baumannii isolates from wild cacao from the Colombian Amazon.World journal of microbiology & biotechnology · 2023Article
- Plant long non-coding RNAs: identification and analysis to unveil their physiological functions.Frontiers in plant science · 2023Review
- Biomolecule-Based Optical Metamaterials: Design and Applications.Biosensors · 2022Review
- DeLTa-Seq: direct-lysate targeted RNA-Seq from crude tissue lysate.Plant methods · 2022Article
- The genetic mechanisms underlying the convergent evolution of pollination syndromes in the Neotropical radiation ofFrontiers in plant science · 2022Article
- Preserving plant samples from remote locations for detection of RNA and DNA viruses.Frontiers in microbiology · 2022Article
- Identification of ABC transporter G subfamily in white lupin and functional characterization of L.albABGC29 in phosphorus use.BMC genomics · 2021Article
- Metagenomic Studies of Viruses in Weeds and Wild Plants: A Powerful Approach to Characterise Variable Virus Communities.Viruses · 2021Review
- Can functional genomic diversity provide novel insights into mechanisms of community assembly? A pilot study from an invaded alpine streambed.Ecology and evolution · 2021Article
- Isolation of high-quality RNA from recalcitrant leaves of variegated and resurrection plants.3 Biotech · 2020Article
- A Combined RNA Preservation and Extraction Protocol for Gene Expression Studies in Cacao Beans.Frontiers in plant science · 2020Article
- Banana (Musa acuminata) transcriptome profiling in response to rhizobacteria: Bacillus amyloliquefaciens Bs006 and Pseudomonas fluorescens Ps006.BMC genomics · 2019Article
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Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledPREMISE OF THE STUDY: To study gene expression in plants, high-quality RNA must be extracted in quantities sufficient for subsequent cDNA library construction. Field-based collections are often limited in quantity and quality of tissue and are typically preserved in RNAlater. Obtaining sufficient and high-quality yield from variously preserved samples is essential to studies of comparative biology. We present a protocol for the extraction of high-quality RNA from even the most recalcitrant plant tissues. • METHODS AND
resultsTissues from mosses, cycads, and angiosperm floral organs and leaves were preserved in RNAlater or frozen fresh at -80°C. Extractions were performed and quality was measured for yield and purity. •
conclusionsThis protocol results in the extraction of high-quality RNA from a variety of plant tissues representing vascular and nonvascular plants. RNA was used for cDNA synthesis to generate libraries for next-generation sequencing and for expression studies using quantitative PCR (qPCR) and semiquantitative reverse transcription PCR (RT-PCR).
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