ArticleApplications in plant sciences2013
Nuclear SSR markers for Miscanthus, Saccharum, and related grasses (Saccharinae, Poaceae).
Article in Applications in plant sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed.
- Assessing Genotypic and Environmental Effects on Endophyte Communities ofJournal of fungi (Basel, Switzerland) · 2021Article
- Genetic diversity and population structure of Miscanthus lutarioriparius, an endemic plant of China.PloS one · 2019Article
- Development of molecular markers for invasive alien plants in Korea: a case study of a toxic weed,PeerJ · 2019Article
- Microsatellite markers: what they mean and why they are so useful.Genetics and molecular biologyArticle
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3 authors.
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Abstract
unlabelledPREMISE OF THE STUDY: We developed nuclear simple sequence repeat (SSR) markers for the characterization of the biomass crop Miscanthus, especially M. sacchariflorus, M. sinensis, and M. ×giganteus, and tested for cross-species amplification. • METHODS AND
resultsTwenty-nine SSR markers (di- and tetranucleotide repeats) were developed from DNA sequences obtained from 192 clones from an enriched genomic library of M. sinensis. All markers were successfully amplified in M. sacchariflorus, M. sinensis, and M. ×giganteus, and 19 amplified across a broad range of Miscanthus species. Polymorphism information content and expected heterozygosity values (19 locus sample) were 0.88 and 0.89, respectively, for M. sinensis, 0.48 and 0.54 for M. sacchariflorus, and were the lowest in M. ×giganteus (0.33, 0.41). Thirteen out of 19 primer pairs showed cross-species amplification in non-Miscanthus sensu stricto taxa. •
conclusionsThe new set of 29 SSR markers will be of high value for characterizing Miscanthus germplasm collections, for prebreeding, and for assessing variation in natural populations.
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