Evidence map›Paper›PMID 25202489›Full record

ArticleApplications in plant sciences2013

Isolation, via 454 sequencing, and characterization of microsatellites for Vachellia farnesiana (Fabaceae: Mimosoideae).

Karen L Bell, Daniel J Murphy, Michael G Gardner

Open access · goldAbstract read
In one paragraph

Article in Applications in plant sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. International journal of molecular sciences · 2023
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 1 country.

Karen L BellRoyal Botanic Gardens Melbourne, Birdwood Avenue, South Yarra, Victoria 3141, Australia ; School of Geography and Environmental Sciences, Monash University, Clayton, Victoria 3800, Australia.
Daniel J MurphyRoyal Botanic Gardens Melbourne, Birdwood Avenue, South Yarra, Victoria 3141, Australia.
Michael G GardnerSchool of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, 5001 South Australia, Australia ; Australian Centre for Evolutionary Biology and Biodiversity, University of Adelaide, Adelaide, 5005 South Australia, Australia ; Evolutionary Biology Unit, South Australian Museum, North Terrace Adelaide, 5000 South Australia, Australia.
Flinders University · AUMonash University · AURoyal Botanic Gardens Victoria

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

unlabelledPREMISE OF THE STUDY: We isolated 15 polymorphic microsatellite markers from Vachellia farnesiana for use in population genetic studies to determine the native range of the species. • METHODS AND

resultsInitially, 454 shotgun sequencing was used to identify and design primers for 68 microsatellite loci. Of these, we trialed 47 loci in the target species, and 42 (89%) amplified a product of expected size. Fifteen of the 47 loci were screened for variation in 21 individuals from the native range of V. farnesiana in southern Mexico and 20 from northwestern Australia. Fourteen loci were polymorphic, with observed heterozygosity ranging from 0.026 to 1.00 (mean = 0.515) and two to 12 alleles per locus (average = 5.2). Cross-amplification was successful in four to 11 loci in three other Vachellia species. •

conclusionsThe new microsatellite loci will be useful in understanding genetic variation and investigating the role of human-mediated dispersal in the current distribution of V. farnesiana.

Indexed as

454 GS-FLXAcacia farnesianacross-species transferabilitymicrosatellitesshotgun sequencingVachellia farnesiana

Identifiers

PMID25202489
PMCPMC4103469
OpenAlexW2169386236

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.