Evidence map›Paper›PMID 25202482›Full record

ArticleApplications in plant sciences2013

Development and characterization of 37 novel EST-SSR markers in Pisum sativum (Fabaceae).

Xiaofeng Zhuang, Kevin E McPhee, Tristan E Coram, Tobin L Peever, Martin I Chilvers

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Article in Applications in plant sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

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4citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Xiaofeng ZhuangDepartment of Plant, Soil and Microbial Sciences, Michigan State University, 107 CIPS, East Lansing, Michigan 48824 USA.
Kevin E McPheeDepartment of Plant Sciences, North Dakota State University, 370G Loftsgard Hall, Fargo, North Dakota 58108 USA.
Tristan E CoramDow AgroSciences LLC, 9330 Zionsville Road, Indianapolis, Indiana 46268 USA.
Tobin L PeeverDepartment of Plant Pathology, Washington State University, Pullman, Washington 99164 USA.
Martin I ChilversDepartment of Plant, Soil and Microbial Sciences, Michigan State University, 107 CIPS, East Lansing, Michigan 48824 USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

unlabelledPREMISE OF THE STUDY: Simple sequence repeat markers were developed based on expressed sequence tags (EST-SSR) and screened for polymorphism among 23 Pisum sativum individuals to assist development and refinement of pea linkage maps. In particular, the SSR markers were developed to assist in mapping of white mold disease resistance quantitative trait loci. • METHODS AND

resultsPrimer pairs were designed for 46 SSRs identified in EST contiguous sequences assembled from a 454 pyrosequenced transcriptome of the pea cultivar, 'LIFTER'. Thirty-seven SSR markers amplified PCR products, of which 11 (30%) SSR markers produced polymorphism in 23 individuals, including parents of recombinant inbred lines, with two to four alleles. The observed and expected heterozygosities ranged from 0 to 0.43 and from 0.31 to 0.83, respectively. •

conclusionsThese EST-SSR markers for pea will be useful for refinement of pea linkage maps, and will likely be useful for comparative mapping of pea and as tools for marker-based pea breeding.

Indexed as

EST-SSRFabaceaemicrosatellitePisum sativumSclerotinia sclerotiorumtranscriptome

Identifiers

PMID25202482
PMCPMC4105355

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