ArticleFrontiers in physiology2014
Microfluidics co-culture systems for studying tooth innervation.
Article in Frontiers in physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- A review of the wide applications of microfluidic devices in peri-implantitis, periodontitis, and endodontics.Odontology · 2026Review
- Organ-on-chip (OoC) and nano-biomaterials: next generation of precision oral and dental healthcare research.Journal of nanobiotechnology · 2026Review
- An Innovative "Tooth-On-Chip" Microfluidic Device Emulating the Structure and Physiology of the Dental Pulp Tissue.Advanced healthcare materials · 2026Article
- Advancing In Vitro Microfluidic Models for Pressure-Induced Retinal Ganglion Cell Degeneration: Current Insights and Future Directions from a Biomechanical Perspective.Micromachines · 2025Article
- Review
- "Periodontal ligament-on-chip" as a Novel Tool for Studies on the Physiology and Pathology of Periodontal Tissues.Advanced healthcare materials · 2024Article
- Dentin Mechanobiology: Bridging the Gap between Architecture and Function.International journal of molecular sciences · 2024Review
- A Systematic Review on Organ-on-a-Chip in PDMS or Hydrogel in Dentistry: An Update of the Literature.Gels (Basel, Switzerland) · 2024Review
- A microfluidic organ-on-a-chip: into the next decade of bone tissue engineering applied in dentistry.Future science OA · 2023Review
- Self-assembled innervated vasculature-on-a-chip to study nociception.Biofabrication · 2023Article
- The Application of Organs-on-a-Chip in Dental, Oral, and Craniofacial Research.Journal of dental research · 2023Review
- Experimental models to study osteoarthritis pain and develop therapeutics.Osteoarthritis and cartilage open · 2022Review
- Cutting-Edge Technologies for Inflamed Joints on Chip: How Close Are We?Frontiers in immunology · 2022Review
- Three-Dimensional Culture Systems for Dissecting Notch Signalling in Health and Disease.International journal of molecular sciences · 2021Review
- Microbiota-Host Immunity Communication in Neurodegenerative Disorders: Bioengineering Challenges for In Vitro Modeling.Advanced healthcare materials · 2021Review
- Article
- A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals.Journal of visualized experiments : JoVE · 2020Article
- Hydrogel Skin-Covered Neurons Self-Assembled with Gustatory Cells for Selective Taste Stimulation.ACS omega · 2019Article
- µSpikeHunter: An advanced computational tool for the analysis of neuronal communication and action potential propagation in microfluidic platforms.Scientific reports · 2019Article
- Advances in ex vivo models and lab-on-a-chip devices for neural tissue engineering.Biomaterials · 2019Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Innervation plays a key role in the development and homeostasis of organs and tissues of the orofacial complex. Among these structures, teeth are peculiar organs as they are not innervated until later stages of development. Furthermore, the implication of neurons in tooth initiation, morphogenesis and differentiation is still controversial. Co-cultures constitute a valuable method to investigate and manipulate the interactions of nerve fibers with their target organs in a controlled and isolated environment. Conventional co-cultures between neurons and their target tissues have already been performed, but these cultures do not offer optimal conditions that are closely mimicking the in vivo situation. Indeed, specific cell populations require different culture media in order to preserve their physiological properties. In this study we evaluate the usefulness of a microfluidics system for co-culturing mouse trigeminal ganglia and developing teeth. This device allows the application of specific media for the appropriate development of both neuronal and dental tissues. The results show that mouse trigeminal ganglia and teeth survive for long culture periods in this microfluidics system, and that teeth maintain the attractive or repulsive effect on trigeminal neurites that has been observed in vivo. Neurites are repealed when co-cultured with embryonic tooth germs, while postnatal teeth exert an attractive effect to trigeminal ganglia-derived neurons. In conclusion, microfluidics system devices provide a valuable tool for studying the behavior of neurons during the development of orofacial tissues and organs, faithfully imitating the in vivo situation.
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