ArticleThe Journal of cell biology2014
Activation of RhoA-ROCK-BMP signaling reprograms adult human corneal endothelial cells.
Article in The Journal of cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- Advances in culture, expansion and mechanistic studies of corneal endothelial cells: a systematic review.Journal of biomedical science · 2019Pooled it
- Protocol for culturing healthy primary endothelial cells isolated from research-grade human corneoscleral donor tissue.STAR protocols · 2026Article
- Corneal Endothelial Progenitors for Ocular Regeneration: Translating Discovery into Clinical Therapies.International journal of molecular sciences · 2026Review
- HC-HA/PTX3 from amniotic membrane reprograms human corneal fibroblasts to neural crest progenitors by switching from canonical to noncanonical TGFβ signaling.Stem cell research & therapy · 2026Article
- Reprogramming of Human Limbal Niche Progenitors.International journal of medical sciences · 2026Review
- Multimodal Imaging of the Corneal Endothelial Transition Zone Reveals Progenitor Cell Population.Cells · 2025Article
- Single-Short Partial Reprogramming of the Endothelial Cells Decreases Blood Pressure via Attenuation of EndMT in Hypertensive Mice.Circulation research · 2025Article
- Substrate Stiffness Modulates Stemness and Differentiation of Rabbit Corneal Endothelium Through the Paxillin-YAP Pathway.Investigative ophthalmology & visual science · 2024Article
- Manufacturing of human corneal endothelial grafts.The ocular surface · 2023Article
- Proliferation Increasing Genetic Engineering in Human Corneal Endothelial Cells: A Literature Review.Frontiers in medicine · 2021Review
- Inorganic phosphate-osteogenic induction medium promotes osteogenic differentiation of valvular interstitial cells via the BMP-2/Smad1/5/9 and RhoA/ROCK-1 signaling pathways.American journal of translational research · 2020Article
- TheInternational journal of medical sciences · 2020Article
- Non-coding RNA in endothelial-to-mesenchymal transition.Cardiovascular research · 2019Review
- Article
- Trabecular meshwork cells are a valuable resource for cellular therapy of glaucoma.Journal of cellular and molecular medicine · 2019Review
- SOX Transcription Factors in Endothelial Differentiation and Endothelial-Mesenchymal Transitions.Frontiers in cardiovascular medicine · 2019Article
- Engineering of Human Corneal Endothelial CellsInternational journal of medical sciences · 2019Review
- Human Trabecular Meshwork Progenitors.International journal of medical sciences · 2019Review
- Cellular Substrates for Cell-Based Tissue Engineering of Human Corneal Endothelial Cells.International journal of medical sciences · 2019Review
- Anticancer Action and Mechanism of Ergosterol Peroxide fromInternational journal of molecular sciences · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Currently there are limited treatment options for corneal blindness caused by dysfunctional corneal endothelial cells. The primary treatment involves transplantation of healthy donor human corneal endothelial cells, but a global shortage of donor corneas necessitates other options. Conventional tissue approaches for corneal endothelial cells are based on EDTA-trypsin treatment and run the risk of irreversible endothelial mesenchymal transition by activating canonical Wingless-related integration site (Wnt) and TGF-β signaling. Herein, we demonstrate an alternative strategy that avoids disruption of cell-cell junctions and instead activates Ras homologue gene family A (RhoA)-Rho-associated protein kinase (ROCK)-canonical bone morphogenic protein signaling to reprogram adult human corneal endothelial cells to neural crest-like progenitors via activation of the miR302b-Oct4-Sox2-Nanog network. This approach allowed us to engineer eight human corneal endothelial monolayers of transplantable size, with a normal density and phenotype from one corneoscleral rim. Given that a similar signal network also exists in the retinal pigment epithelium, this partial reprogramming approach may have widespread relevance and potential for treating degenerative diseases.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.