ArticleProceedings of the National Academy of Sciences of the United States of America2014
Biophysical implications of lipid bilayer rheometry for mechanosensitive channels.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 68 citations in OpenAlex.
- A two-step clockwork mechanism opens a proteo-lipidic pore in PIEZO2.Nature chemical biology · 2026Article
- Opening threshold and kinetics of the MscL mechanosensitive channel are regulated by its periplasmic loop.Research square · 2026Article
- Opening threshold and kinetics of the MscL mechanosensitive channel are regulated by its periplasmic loop.npj biological physics and mechanics · 2026Article
- Advancements in Engineering Planar Model Cell Membranes: Current Techniques, Applications, and Future Perspectives.Nanomaterials (Basel, Switzerland) · 2024Review
- Microscale geometrical modulation of PIEZO1 mediated mechanosensing through cytoskeletal redistribution.Nature communications · 2024Article
- "Force-From-Lipids" Dependence of the MscCG Mechanosensitive Channel Gating on Anionic Membranes.Microorganisms · 2023Article
- Capsaicin as an amphipathic modulator of NaChannels (Austin, Tex.) · 2022Article
- Membrane Stretch Gates NMDA Receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Membrane Protein Activity Induces Specific Molecular Changes in Nanodiscs Monitored by FTIR Difference Spectroscopy.Frontiers in molecular biosciences · 2022Article
- Effects of Nanopillar Size and Spacing on Mechanical Perturbation and Bactericidal Killing Efficiency.Nanomaterials (Basel, Switzerland) · 2021Article
- Mechanical properties of ester- and ether-DPhPC bilayers: A molecular dynamics study.Journal of the mechanical behavior of biomedical materials · 2021Article
- Mechanics of Bacterial Interaction and Death on Nanopatterned Surfaces.Biophysical journal · 2021Article
- Review
- Coarse-Grained Modeling of Pore Dynamics on the Red Blood Cell Membrane under Large Deformations.Biophysical journal · 2020Article
- Localized Axolemma Deformations Suggest Mechanoporation as Axonal Injury Trigger.Frontiers in neurology · 2020Article
- Piezo1 mechanosensitive channels: what are they and why are they important.Biophysical reviews · 2019Review
- Corynebacterium glutamicum mechanosensitive channels: towards unpuzzling "glutamate efflux" for amino acid production.Biophysical reviews · 2018Review
- Tuning ion channel mechanosensitivity by asymmetry of the transbilayer pressure profile.Biophysical reviews · 2018Review
- Evolutionary specialization of MscCG, an MscS-like mechanosensitive channel, in amino acid transport in Corynebacterium glutamicum.Scientific reports · 2018Article
- β1 subunit stabilises sodium channel Nav1.7 against mechanical stress.The Journal of physiology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The lipid bilayer plays a crucial role in gating of mechanosensitive (MS) channels. Hence it is imperative to elucidate the rheological properties of lipid membranes. Herein we introduce a framework to characterize the mechanical properties of lipid bilayers by combining micropipette aspiration (MA) with theoretical modeling. Our results reveal that excised liposome patch fluorometry is superior to traditional cell-attached MA for measuring the intrinsic mechanical properties of lipid bilayers. The computational results also indicate that unlike the uniform bilayer tension estimated by Laplace's law, bilayer tension is not uniform across the membrane patch area. Instead, the highest tension is seen at the apex of the patch and the lowest tension is encountered near the pipette wall. More importantly, there is only a negligible difference between the stress profiles of the outer and inner monolayers in the cell-attached configuration, whereas a substantial difference (∼30%) is observed in the excised configuration. Our results have far-reaching consequences for the biophysical studies of MS channels and ion channels in general, using the patch-clamp technique, and begin to unravel the difference in activity seen between MS channels in different experimental paradigms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.