ArticleProceedings of the National Academy of Sciences of the United States of America2014
Channelrhodopsin-2-XXL, a powerful optogenetic tool for low-light applications.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 107 papers.
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Who cites it
107 citing papers in PubMed.
- Article
- Selective octopaminergic tuning of mushroom body circuits during memory formation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Stabilized Ion Selectivity Corrects Activation Drift in Kalium Channelrhodopsins.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Entirely synthetic memory inception via dual optogenetic activation of sensory and dopaminergic neurons.iScience · 2025Article
- Monolithic multimodal neural probes for sustained stimulation and long-term neural recording.Science advances · 2025Article
- Two Decades of Optogenetic Tools: A Retrospective and a Look Ahead.Advanced genetics (Hoboken, N.J.) · 2025Review
- Neuropeptide dynamics coordinate layered plasticity mechanisms adaptingScience advances · 2025Article
- Effect of Retinal on Dictyostelium Cells During Development.Genes to cells : devoted to molecular & cellular mechanisms · 2025Article
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- Compromising Tyrosine Hydroxylase Function Extends and Blunts the Temporal Profile of Reinforcement by Dopamine Neurons inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Suppression of epileptic seizures by transcranial activation of KNature communications · 2025Article
- Optogenetic control ofJournal of microbiology & biology education · 2024Article
- Activity Monitoring for Analysis of Sleep inCold Spring Harbor protocols · 2024Article
- Characterization of the tail current of Channelrhodopsin-2 variants.Biochemistry and biophysics reports · 2024Article
- Article
- Expression and potential regulatory functions of Drosophila octopamine receptors in the female reproductive tract.G3 (Bethesda, Md.) · 2024Article
- A micro-LED array based platform for spatio-temporal optogenetic control of various cardiac models.Scientific reports · 2023Article
- Rewarding Capacity of Optogenetically Activating a Giant GABAergic Central-Brain Interneuron in LarvalThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- OptoPi: An open source flexible platform for the analysis of small animal behaviour.HardwareX · 2023Article
- Article
47 more citing papers are in PubMed but not listed here.
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11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Channelrhodopsin-2 (ChR2) has provided a breakthrough for the optogenetic control of neuronal activity. In adult Drosophila melanogaster, however, its applications are severely constrained. This limitation in a powerful model system has curtailed unfolding the full potential of ChR2 for behavioral neuroscience. Here, we describe the D156C mutant, termed ChR2-XXL (extra high expression and long open state), which displays increased expression, improved subcellular localization, elevated retinal affinity, an extended open-state lifetime, and photocurrent amplitudes greatly exceeding those of all heretofore published ChR variants. As a result, neuronal activity could be efficiently evoked with ambient light and even without retinal supplementation. We validated the benefits of the variant in intact flies by eliciting simple and complex behaviors. We demonstrate efficient and prolonged photostimulation of monosynaptic transmission at the neuromuscular junction and reliable activation of a gustatory reflex pathway. Innate male courtship was triggered in male and female flies, and olfactory memories were written through light-induced associative training.
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