ArticleJournal of bacteriology2014
Assembling flagella in Salmonella mutant strains producing a type III export apparatus without FliO.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- FliO is an evolutionarily conserved yet diversified core component of the bacterial flagellar type III secretion system.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Suppressor Mutants: History and Today's Applications.EcoSal Plus · 2021Review
- Molecular and Cell Biological Analysis of SwrB in Bacillus subtilis.Journal of bacteriology · 2021Article
- Integration of the pSLT Plasmid into theJournal of bacteriology · 2020Article
- Studies on expression levels of3 Biotech · 2020Article
- The Flagellar Gene Regulates Biofilm Formation and Mussel Larval Settlement and Metamorphosis.International journal of molecular sciences · 2020Article
- Beyond a Ribosomal RNA Methyltransferase, the Wider Role of MraW in DNA Methylation, Motility and Colonization inFrontiers in microbiology · 2019Article
- Connecting single-cell properties to collective behavior in multiple wild isolates of the Enterobacter cloacae complex.PloS one · 2019Article
- Evaluating the potential of residual Pap test fluid as a resource for the metaproteomic analysis of the cervical-vaginal microbiome.Scientific reports · 2018Article
- A flagellum-specific chaperone facilitates assembly of the core type III export apparatus of the bacterial flagellum.PLoS biology · 2017Article
- Assembly and stoichiometry of the core structure of the bacterial flagellar type III export gate complex.PLoS biology · 2017Article
- Type III secretion systems: the bacterial flagellum and the injectisome.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Functional Activation of the Flagellar Type III Secretion Export Apparatus.PLoS genetics · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The type III export apparatus of the Salmonella flagellum consists of six transmembrane proteins (FlhA, FlhB, FliO, FliP, FliQ, and FliR) and three soluble proteins (FliH, FliI, and FliJ). Deletion of the fliO gene creates a mutant strain that is poorly motile; however, suppressor mutations in the fliP gene can partially rescue motility. To further understand the mechanism of suppression of a fliO deletion mutation, we isolated new suppressor mutant strains with partially rescued motility. Whole-genome sequence analysis of these strains found a missense mutation that localized to the clpP gene [clpP(V20F)], which encodes the ClpP subunit of the ClpXP protease, and a synonymous mutation that localized to the fliA gene [fliA(+36T→C)], which encodes the flagellar sigma factor, σ(28). Combining these suppressor mutations with mutations in the fliP gene additively rescued motility and biosynthesis of the flagella in fliO deletion mutant strains. Motility was also rescued by an flgM deletion mutation or by plasmids carrying either the flhDC or fliA gene. The fliA(+36T→C) mutation increased mRNA translation of a fliA'-lacZ gene fusion, and immunoblot analysis revealed that the mutation increased levels of σ(28). Quantitative real-time reverse transcriptase PCR showed that either the clpP(V20F) or fliA(+36T→C) mutation rescued expression of class 3 flagellar and chemotaxis genes; still, the suppressor mutations in the fliP gene had a greater effect on bypassing the loss of fliO function. This suggests that the function of FliO is closely associated with regulation of FliP during assembly of the flagellum.
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