ArticleJournal of bacteriology2014
A CRISPR with roles in Myxococcus xanthus development and exopolysaccharide production.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Characterization ofmSphere · 2024Article
- MyxoPortal: a database of myxobacterial genomic features.Database : the journal of biological databases and curation · 2024Article
- Characteristics and immune functions of the endogenous CRISPR-Cas systems in myxobacteria.mSystems · 2024Article
- Article
- Whole-Genome Sequence ofMicrobiology resource announcements · 2023Article
- Diversity of Myxobacteria Isolated from Indonesian Mangroves and Their Potential for New Antimicrobial Sources.Current microbiology · 2022Article
- Alternative functions of CRISPR-Cas systems in the evolutionary arms race.Nature reviews. Microbiology · 2022Review
- Digging into the lesser-known aspects of CRISPR biology.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Review
- Complete Genome Sequence ofMicrobiology resource announcements · 2021Article
- The CRISPR-Cas System Is Involved in OmpR Genetic Regulation for Outer Membrane Protein Synthesis inFrontiers in microbiology · 2021Article
- Characterization of the Exopolysaccharide Biosynthesis Pathway in Myxococcus xanthus.Journal of bacteriology · 2020Article
- The CRISPR-Cas Mechanism for Adaptive Immunity and Alternate Bacterial Functions Fuels Diverse Biotechnologies.Frontiers in cellular and infection microbiology · 2020Review
- CRISPR-Cas Systems and the Paradox of Self-Targeting Spacers.Frontiers in microbiology · 2019Review
- Multifactorial control of the expression of a CRISPR-Cas system by an extracytoplasmic function σ/anti-σ pair and a global regulatory complex.Nucleic acids research · 2018Article
- Bacteriophages ofViruses · 2018Review
- The type IV pilus assembly ATPase PilB functions as a signaling protein to regulate exopolysaccharide production in Myxococcus xanthus.Scientific reports · 2017Article
- Identification of Functions Affecting Predator-Prey Interactions between Myxococcus xanthus and Bacillus subtilis.Journal of bacteriology · 2016Article
- Overview of CRISPR-Cas9 Biology.Cold Spring Harbor protocols · 2016Article
- CRISPR-Cas: biology, mechanisms and relevance.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- A barrier to homologous recombination between sympatric strains of the cooperative soil bacterium Myxococcus xanthus.The ISME journal · 2016Article
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4 authors.
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Abstract
The Gram-negative soil bacterium Myxococcus xanthus utilizes its social (S) gliding motility to move on surfaces during its vegetative and developmental cycles. It is known that S motility requires the type IV pilus (T4P) and the exopolysaccharide (EPS) to function. The T4P is the S motility motor, and it powers cell movement by retraction. As the key regulator of the S motor, EPS is proposed to be the anchor and trigger for T4P retraction. The production of EPS is regulated in turn by the T4P in M. xanthus, and T4P(-) mutants are S(-) and EPS(-). In this study, a ΔpilA strain (T4P(-) and EPS(-)) was mutagenized by a transposon and screened for EPS(+) mutants. A pilA suppressor isolated as such harbored an insertion in the 3rd clustered regularly interspaced short palindromic repeat (CRISPR3) in M. xanthus. Evidence indicates that this transposon insertion, designated CRISPR3*, is a gain-of-function (GOF) mutation. Moreover, CRISPR3* eliminated developmental aggregation in both the wild-type and the pilA mutant backgrounds. Upstream of CRISPR3 are genes encoding the repeat-associated mysterious proteins (RAMPs). These RAMP genes are indispensable for CRISPR3* to affect development and EPS in M. xanthus. Analysis by reverse transcription (RT)-PCR suggested that CRISPR3* led to an increase in the processing of the RNA transcribed from CRISPR3. We propose that certain CRISPR3 transcripts, once expressed and processed, target genes critical for M. xanthus fruiting body development and EPS production in a RAMP-dependent manner.
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