ArticleJournal of dental research2014
Scaffold-free Prevascularized Microtissue Spheroids for Pulp Regeneration.
Article in Journal of dental research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
61 citing papers in PubMed, 141 citations in OpenAlex.
- Strategies to Microengineer Vascularized, Mineralized, and Immunomodulatory Niches for Dental Regeneration.Journal of endodontics · 2026Article
- Regenerative Dentistry: Applications of Bioactive Materials in Dentin-Pulp Complex.Advances in experimental medicine and biology · 2026Review
- Three-Dimensional Models of the Dental Pulp: Bridging Fundamental Biology and Regenerative Therapy.International journal of molecular sciences · 2025Review
- EphrinB2 in dental pulp stem cells promotes endothelial cells forming capillary-like cords in a 3D bioprinted hydrogel construct.BMC molecular and cell biology · 2025Article
- Optimizing methods for regenerative endodontics.Regenerative medicine · 2025Review
- Regenerative endodontic therapy: From laboratory bench to clinical practice.Journal of advanced research · 2025Review
- Guidance for evaluating biomaterials' properties and biological potential for dental pulp tissue engineering and regeneration research.Dental materials : official publication of the Academy of Dental Materials · 2025Review
- Scaffold-Free Strategies in Dental Pulp/Dentine Tissue Engineering: Current Status and Implications for Regenerative Biological Processes.Bioengineering (Basel, Switzerland) · 2025Review
- Multiscale Imaging to Monitor Functional SHED-Supported Engineered Vessels.Journal of dental research · 2024Article
- B2M or CIITA knockdown decreased the alloimmune response of dental pulp stem cells: an in vitro study.Stem cell research & therapy · 2024Article
- Bone Spheroid Development Under Flow Conditions with Mesenchymal Stem Cells and Human Umbilical Vein Endothelial Cells in a 3D Porous Hydrogel Supplemented with Hydroxyapatite.Gels (Basel, Switzerland) · 2024Article
- Dual roles of photosynthetic hydrogel with sustained oxygen generation in promoting cell survival and eradicating anaerobic infection.Materials today. Bio · 2024Article
- Patterned PVA Hydrogels with 3D Petri DishGels (Basel, Switzerland) · 2024Article
- Bioengineering from the laboratory to clinical translation in oral and maxillofacial reconstruction.The Saudi dental journal · 2024Review
- Identifying Dental Pulp Stem Cell as a Novel Therapeutic trategy for Digestive Diseases.Current stem cell research & therapy · 2024Review
- Vascularized Tissue Organoids.Bioengineering (Basel, Switzerland) · 2023Review
- Creating a Microenvironment to Give Wings to Dental Pulp Regeneration-Bioactive Scaffolds.Pharmaceutics · 2023Review
- Therapeutic strategies of three-dimensional stem cell spheroids and organoids for tissue repair and regeneration.Bioactive materials · 2023Review
- Prevascularization techniques for dental pulp regeneration: potential cell sources, intercellular communication and construction strategies.Frontiers in bioengineering and biotechnology · 2023Review
- Decellularized rat submandibular gland as an alternative scaffold for dental pulp regeneration.Frontiers in bioengineering and biotechnology · 2023Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Creating an optimal microenvironment that mimics the extracellular matrix (ECM) of natural pulp and securing an adequate blood supply for the survival of cell transplants are major hurdles that need to be overcome in dental pulp regeneration. However, many currently available scaffolds fail to mimic essential functions of natural ECM. The present study investigated a novel approach involving the use of scaffold-free microtissue spheroids of dental pulp stem cells (DPSCs) prevascularized by human umbilical vein endothelial cells (HUVECs) in pulp regeneration. In vitro-fabricated microtissue spheroids were inserted into the canal space of tooth-root slices and were implanted subcutaneously into immunodeficient mice. Histological examination revealed that, after four-week implantation, tooth-root slices containing microtissue spheroids resulted in well-vascularized and cellular pulp-like tissues, compared with empty tooth-root slices, which were filled with only subcutaneous fat tissue. Immunohistochemical staining indicated that the tissue found in the tooth-root slices was of human origin, as characterized by the expression of human mitochondria, and contained odontoblast-like cells organized along the dentin, as assessed by immunostaining for nestin and dentin sialoprotein (DSP). Vascular structures formed by HUVECs in vitro were successfully anastomosed with the host vasculature upon transplantation in vivo, as shown by immunostaining for human CD31. Collectively, these findings demonstrate that prevascularized, scaffold-free, microtissue spheroids can successfully regenerate vascular dental pulp-like tissue and also highlight the significance of the microtissue microenvironment as an optimal environment for successful pulp-regeneration strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.