ArticleJournal of the American Society of Nephrology : JASN2015
Functional genetic targeting of embryonic kidney progenitor cells ex vivo.
Article in Journal of the American Society of Nephrology : JASN, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- FromLab on a chip · 2024Article
- Vascularization of kidney organoids: different strategies and perspectives.Frontiers in urology · 2024Review
- Generation of novel in vitro flexible kidney organoid model to investigate the role of extracellular vesicles in induction of nephrogenesis.Cell communication and signaling : CCS · 2023Article
- Production of kidney organoids arranged around single ureteric bud trees, and containing endogenous blood vessels, solely from embryonic stem cells.Scientific reports · 2022Article
- Article
- Kidney Regeneration in Later-Stage Mouse EmbryosJournal of the American Society of Nephrology : JASN · 2019Article
- Simple 3D culture of dissociated kidney mesenchyme mimics nephron progenitor niche and facilitates nephrogenesis Wnt-independently.Scientific reports · 2019Article
- Embryonic Stem Cells Derived Kidney Organoids as Faithful Models to Target Programmed Nephrogenesis.Scientific reports · 2018Article
- The multisystemic functions of FOXD1 in development and disease.Journal of molecular medicine (Berlin, Germany) · 2018Review
- Exosomes as secondary inductive signals involved in kidney organogenesis.Journal of extracellular vesicles · 2018Article
- Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy.PloS one · 2018Article
- Troy/TNFRSF19 marks epithelial progenitor cells during mouse kidney development that continue to contribute to turnover in adult kidney.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Renal carcinoma/kidney progenitor cell chimera organoid as a novel tumorigenesis gene discovery model.Disease models & mechanisms · 2017Article
- Endothelial marker-expressing stromal cells are critical for kidney formation.American journal of physiology. Renal physiology · 2017Article
- Novel fixedDevelopment (Cambridge, England) · 2017Article
- New insights into precursors of renal endothelium.Kidney international · 2016Article
- cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness.Scientific reports · 2016Article
- Wnt Signaling in Renal Cell Carcinoma.Cancers · 2016Review
- Beyond Turing: mechanochemical pattern formation in biological tissues.Biology direct · 2016Article
- Mechanochemical symmetry breaking in Hydra aggregates.Biophysical journal · 2015Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 4 countries.
Funding
Abstract
The embryonic mammalian metanephric mesenchyme (MM) is a unique tissue because it is competent to generate the nephrons in response to Wnt signaling. An ex vivo culture in which the MM is separated from the ureteric bud (UB), the natural inducer, can be used as a classic tubule induction model for studying nephrogenesis. However, technological restrictions currently prevent using this model to study the molecular genetic details before or during tubule induction. Using nephron segment-specific markers, we now show that tubule induction in the MM ex vivo also leads to the assembly of highly segmented nephrons. This induction capacity was reconstituted when MM tissue was dissociated into a cell suspension and then reaggregated (drMM) in the presence of human recombinant bone morphogenetic protein 7/human recombinant fibroblast growth factor 2 for 24 hours before induction. Growth factor-treated drMM also recovered the capacity for organogenesis when recombined with the UB. Cell tracking and time-lapse imaging of chimeric drMM cultures indicated that the nephron is not derived from a single progenitor cell. Furthermore, viral vector-mediated transduction of green fluorescent protein was much more efficient in dissociated MM cells than in intact mesenchyme, and the nephrogenic competence of transduced drMM progenitor cells was preserved. Moreover, drMM cells transduced with viral vectors mediating Lhx1 knockdown were excluded from the nephric tubules, whereas cells transduced with control vectors were incorporated. In summary, these techniques allow reproducible cellular and molecular examinations of the mechanisms behind nephrogenesis and kidney organogenesis in an ex vivo organ culture/organoid setting.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.