Evidence map›Paper›PMID 25201686›Full record

ArticleCurrent biology : CB2014

Tcf7l2 is required for left-right asymmetric differentiation of habenular neurons.

Ulrike Hüsken, Heather L Stickney, Gaia Gestri, Isaac H Bianco, Ana Faro, Rodrigo M Young, Myriam Roussigne, Thomas A Hawkins, Carlo A Beretta, Irena Brinkmann and 17 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current biology : CB, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 64 citations in OpenAlex.

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  11. Conserved and diverged asymmetric gene expression in the brain of teleosts.Frontiers in cell and developmental biology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

27 authors at 7 institutions in 6 countries.

Ulrike HüskenDepartment of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg University, Ludolf-Krehl-Strasse 13-17, 68167 Mannheim, Germany.
Heather L StickneyDepartment of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
Gaia GestriDepartment of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
Isaac H BiancoDepartment of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
Ana FaroDepartment of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
Rodrigo M YoungDepartment of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
Myriam RoussigneDepartment of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK; Centre de Biologie du Développement (CDB), UPS, Université de Toulouse, 118 Route de Narbonne, 31062, France; CNRS, CDB UMR 5547, 31062 Toulouse, France.
Thomas A HawkinsDepartment of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
Carlo A BerettaDepartment of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg University, Ludolf-Krehl-Strasse 13-17, 68167 Mannheim, Germany.
Irena BrinkmannDepartment of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg University, Ludolf-Krehl-Strasse 13-17, 68167 Mannheim, Germany.
Alessio PaoliniDepartment of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg University, Ludolf-Krehl-Strasse 13-17, 68167 Mannheim, Germany.
Raquel JacintoDepartment of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg University, Ludolf-Krehl-Strasse 13-17, 68167 Mannheim, Germany.
Shahad AlbadriCentre for Organismal Studies, Heidelberg University, 69120 Heidelberg, Germany.
Elena DreostiDepartment of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK; Neuroelectronics Research Flanders, 3001 Leuven, Belgium.
Matina TsalavoutaDepartment of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
Quenten SchwarzInstitute of Ophthalmology, University College London, London EC1V 9EL, UK.
Florencia CavodeassiDepartment of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
Anukampa K BarthDepartment of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
Lu WenKey Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China.
Bo ZhangKey Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China.
Patrick BladerCentre de Biologie du Développement (CDB), UPS, Université de Toulouse, 118 Route de Narbonne, 31062, France; CNRS, CDB UMR 5547, 31062 Toulouse, France.
Emre YaksiNeuroelectronics Research Flanders, 3001 Leuven, Belgium; Vlaams Instituut voor Biotechnologie, 3001 Leuven, Belgium; KU Leuven, 3001 Leuven, Belgium.
Lucia PoggiCentre for Organismal Studies, Heidelberg University, 69120 Heidelberg, Germany.
Mihaela ZigmanDepartment of Molecular Evolution and Genomics, Centre for Organismal Studies (COS), Heidelberg University, Im Neuenheimer Feld 329, 69120 Heidelberg, Germany.
Shuo LinKey Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China; Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, 621 Charles E. Young Drive, Los Angeles, CA 90095, USA.
Stephen W WilsonDepartment of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK. Electronic address: s.wilson@ucl.ac.uk.
Matthias CarlDepartment of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg University, Ludolf-Krehl-Strasse 13-17, 68167 Mannheim, Germany. Electronic address: matthias.carl@medma.uni-heidelberg.de.
University College London · GBHeidelberg University · DEPeking University · CNUniversity Hospital Heidelberg · DECentre National de la Recherche Scientifique · FRKU Leuven · BEUniversity Medical Centre Mannheim · DE

Funding

Biotechnology and Biological Sciences Research Council BB/H012516/1Medical Research Council MR/L003775/1Wellcome Trust
6 · The paper itself

Abstract

backgroundAlthough left-right asymmetries are common features of nervous systems, their developmental bases are largely unknown. In the zebrafish epithalamus, dorsal habenular neurons adopt medial (dHbm) and lateral (dHbl) subnuclear character at very different frequencies on the left and right sides. The left-sided parapineal promotes the elaboration of dHbl character in the left habenula, albeit by an unknown mechanism. Likewise, the genetic pathways acting within habenular neurons to control their asymmetric differentiated character are unknown.

resultsIn a forward genetic screen for mutations that result in loss of habenular asymmetry, we identified two mutant alleles of tcf7l2, a gene that encodes a transcriptional regulator of Wnt signaling. In tcf7l2 mutants, most neurons on both sides differentiate with dHbl identity. Consequently, the habenulae develop symmetrically, with both sides adopting a pronounced leftward character. Tcf7l2 acts cell automously in nascent equipotential neurons, and on the right side, it promotes dHbm and suppresses dHbl differentiation. On the left, the parapineal prevents this Tcf7l2-dependent process, thereby promoting dHbl differentiation.

conclusionsTcf7l2 is essential for lateralized fate selection by habenular neurons that can differentiate along two alternative pathways, thereby leading to major neural circuit asymmetries.

Indexed as

Cell DifferentiationAnimalsEmbryo, NonmammalianGene Expression RegulationHabenulaNeuronsSignal TransductionTranscription Factor 7-Like 2 ProteinZebrafishZebrafish ProteinsTCF7L2 protein, zebrafishTranscription Factor 7-Like 2 ProteinZebrafish Proteins

Identifiers

PMID25201686
PMCPMC4194317
OpenAlexW2072885793

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.