Evidence map›Paper›PMID 25200612›Full record

ArticleNanomedicine : nanotechnology, biology, and medicine2015

Cellular level robotic surgery: Nanodissection of intermediate filaments in live keratinocytes.

Ruiguo Yang, Bo Song, Zhiyong Sun, King Wai Chiu Lai, Carmen Kar Man Fung, Kevin C Patterson, Kristina Seiffert-Sinha, Animesh A Sinha, Ning Xi

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nanomedicine : nanotechnology, biology, and medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.3field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 38 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Nanosurgical Manipulation of Titin and Its M-Complex.Nanomaterials (Basel, Switzerland) · 2022
    Article
  6. Advanced tools and methods for single-cell surgery.Microsystems & nanoengineering · 2022
    Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 3 countries.

Ruiguo YangDepartment of Electrical and Computer Engineering, Michigan State University, East Lansing, MI, 48824, USA.
Bo SongDepartment of Electrical and Computer Engineering, Michigan State University, East Lansing, MI, 48824, USA.
Zhiyong SunDepartment of Electrical and Computer Engineering, Michigan State University, East Lansing, MI, 48824, USA.
King Wai Chiu LaiDepartment of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong.
Carmen Kar Man FungHong Kong Productivity Council, Hong Kong.
Kevin C PattersonCollege of Human Medicine, Michigan State University, East Lansing, MI, 48824, USA.
Kristina Seiffert-SinhaDepartment of Dermatology, University at Buffalo, Buffalo, NY, 14203, USA.
Animesh A SinhaDepartment of Dermatology, University at Buffalo, Buffalo, NY, 14203, USA. Electronic address: aasinha@buffalo.edu.
Ning XiDepartment of Electrical and Computer Engineering, Michigan State University, East Lansing, MI, 48824, USA. Electronic address: xin@egr.msu.edu.
Michigan State University · USBuffalo State University · USCity University of Hong Kong · HKHong Kong Productivity Council · CNUniversity at Buffalo, State University of New York · US

Funding

Novel single cell disease markers with a hybrid AFM scanning piezo-thermal probeR43GM084520 · NIGMS · KYTARO, INC. · PI GAITAS, ANGELO · 2008 to 2009
$400k
NIGMS NIH HHS R43 GM084520
6 · The paper itself

Abstract

We present the nanosurgery on the cytoskeleton of live cells using AFM based nanorobotics to achieve adhesiolysis and mimic the effect of pathophysiological modulation of intercellular adhesion. Nanosurgery successfully severs the intermediate filament bundles and disrupts cell-cell adhesion similar to the desmosomal protein disassembly in autoimmune disease, or the cationic modulation of desmosome formation. Our nanomechanical analysis revealed that adhesion loss results in a decrease in cellular stiffness in both cases of biochemical modulation of the desmosome junctions and mechanical disruption of intercellular adhesion, supporting the notion that intercellular adhesion through intermediate filaments anchors the cell structure as focal adhesion does and that intermediate filaments are integral components in cell mechanical integrity. The surgical process could potentially help reveal the mechanism of autoimmune pathology-induced cell-cell adhesion loss as well as its related pathways that lead to cell apoptosis.

Indexed as

RoboticsApoptosisAutoimmune DiseasesCationsCell AdhesionCell LineCytoskeletonDesmosomesHumansIntermediate FilamentsKeratinocytesMicroscopy, Atomic ForceNanomedicineNanostructuresStress, MechanicalSurgery, Computer-AssistedCationsAtomic Force MicroscopyCell–cell adhesionDesmosomeIntermediate filamentMechanical propertyNanosurgery

Identifiers

PMID25200612
PMCPMC4280342
OpenAlexW1987601706

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.