ArticleBMC veterinary research2014
Rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction.
Article in BMC veterinary research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 45 citations in OpenAlex.
- Circulation of arctic canine distemper virus in diarrheic dogs in Iran: molecular detection and phylogenetic characterization.BMC research notes · 2026Article
- Development and validation of a reverse transcription-insulated isothermal PCR assay for detection of caprine kobuvirus and its epidemiological and molecular characterization in China.Frontiers in veterinary science · 2026Article
- Investigation of the canine distemper virus in dogs living at public kennels in Northern Brazil: validation of a detection assay.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) designed for rapid detection of canine distemper virus.The Journal of veterinary medical science · 2024Article
- Development and validation of an insulated isothermal polymerase chain reaction assay for the rapid detection ofVeterinary research forum : an international quarterly journal · 2024Article
- Advanced Strategies for Developing Vaccines and Diagnostic Tools for African Swine Fever.Viruses · 2023Review
- Canine morbillivirus (CDV): a review on current status, emergence and the diagnostics.Virusdisease · 2022Review
- Development and validation of an insulated isothermal PCR assay for the rapid detection ofJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2022Article
- Critical Problems for Research in Animal Sheltering, a Conceptual Analysis.Frontiers in veterinary science · 2022Review
- Development and validation of a portable, point-of-care canine distemper virus qPCR test.PloS one · 2020Article
- Development and evaluation of a real-time RT-PCR and a field-deployable RT-insulated isothermal PCR for the detection of Seneca Valley virus.BMC veterinary research · 2019Article
- Detection and characterisation of canine astrovirus, canine parvovirus and canine papillomavirus in puppies using next generation sequencing.Scientific reports · 2019Article
- Evaluation of an incubation instrument-free reverse transcription recombinase polymerase amplification assay for rapid and point-of-need detection of canine distemper virus.Journal of virological methods · 2018Article
- Validation of the Pockit Dengue Virus Reagent Set for Rapid Detection of Dengue Virus in Human Serum on a Field-Deployable PCR System.Journal of clinical microbiology · 2018Article
- Rapid and sensitive insulated isothermal PCR for point-of-need feline leukaemia virus detection.Journal of feline medicine and surgery · 2018Article
- Rapid Diagnosis of Babesia gibsoni by Point-of-Need Testing by Insulated Isothermal PCR in Dogs at High Risk of Infection.Journal of veterinary internal medicine · 2018Article
- Evaluation of a field-deployable reverse transcription-insulated isothermal PCR for rapid and sensitive on-site detection of Zika virus.BMC infectious diseases · 2017Article
- Development of real-time recombinase polymerase amplification assay for rapid and sensitive detection of canine parvovirus 2.BMC veterinary research · 2017Article
- Evaluation and Clinical Validation of Two Field-Deployable Reverse Transcription-Insulated Isothermal PCR Assays for the Detection of the Middle East Respiratory Syndrome-Coronavirus.The Journal of molecular diagnostics : JMD · 2017Article
- Field-Deployable Reverse Transcription-Insulated Isothermal PCR (RT-iiPCR) Assay for Rapid and Sensitive Detection of Foot-and-Mouth Disease Virus.Transboundary and emerging diseases · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCanine distemper virus (CDV) has been associated with outbreaks of canine infectious respiratory disease in shelters and boarding kennel environments. POCKITTM Nucleic Acid Analyzer is a field-deployable device capable of generating automatically interpreted insulated isothermal polymerase chain reaction (iiPCR) results from extracted nucleic acid within one hour. In this study, reverse transcription iiPCR (RT-iiPCR) was developed to facilitate point-of-need diagnosis of CDV infection.
resultsAnalytical sensitivity (limit of detection 95%) of the established CDV RT-iiPCR was about 11 copies of in vitro transcribed RNA per reaction. CDV RT-iiPCR generated positive signals from CDV, but not Bordetella bronchiseptica, canine parvovirus, canine herpesvirus, canine adenovirus 2, canine influenza virus (subtype H3N8), canine parainfluenza virus, and canine respiratory coronavirus. To evaluate accuracy of the established reaction in canine distemper clinical diagnosis, 110 specimens from dogs, raccoons, and foxes suspected with CDV infection were tested simultaneously by CDV RT-iiPCR and real-time RT-PCR. CDV RT-iiPCR demonstrated excellent sensitivity (100%) and specificity (100%), compared to real-time RT-PCR.
conclusionsThe results indicated an excellent correlation between RT-iiPCR and a reference real time RT-PCR method. Working in a lyophilized format, the established method has great potential to be used for point-of-care diagnosis of canine distemper in animals, especially in resource-limited facilities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.