ArticleBMC pediatrics2014
Comparative study of bacteriological culture and real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay in the diagnosis of bacterial neonatal meningitis.
Article in BMC pediatrics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 54 citations in OpenAlex.
- A Molecular Approach for Detecting Bacteria and Fungi in Healthcare Environment Aerosols: A Systematic Review.International journal of molecular sciences · 2024Pooled it
- Diagnostic Performance of Complementary Serum and Cerebrospinal Fluid Multiplex PCR for Acute Bacterial Meningitis.Diagnostics (Basel, Switzerland) · 2026Article
- Etiology of invasive bacterial infections in infants 90 days and younger presenting to the emergency department in a children's hospital in Saudi Arabia.BMC infectious diseases · 2026Article
- A novel real-time PCR assay for the simultaneous detection of the four main causes of bacterial meningitis.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2026Article
- Enhanced detection of bacterial etiologies, antibiotic resistance profile, and treatment outcomes of meningitis patients in Ethiopia: a prospective cross-sectional study.BMC microbiology · 2025Article
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- Clinical, Laboratory Characteristics, and Microorganism Infection Status in Neonates With Meningitis in Vietnam: A Cross-Sectional Descriptive Study.International journal of pediatrics · 2025Article
- Molecular diagnostic methods for rapid diagnosis of central nervous system infections.Frontiers in medical technology · 2025Review
- Improved Diagnostics in Bacterial Neonatal Meningitis Using a Next-Generation Sequencing Platform.Infectious diseases and therapy · 2023Article
- Clinical use of multiplex-PCR for the diagnosis of acute bacterial meningitis.Journal of family medicine and primary care · 2022Article
- Coronavirus Disease 2019 (COVID-19): Emerging detection technologies and auxiliary analysis.Journal of clinical laboratory analysis · 2022Review
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- Multiplex PCR method for simultaneous detection of five pathogenic bacteria closely related to foodborne diseases.3 Biotech · 2021Article
- Detection ofInfectious disease reports · 2021Article
- Antimicrobial Resistance Pattern of Bacterial Meningitis Among Patients in Quetta, Pakistan.Infection and drug resistance · 2021Article
- Impact of Laboratory Methods and Gene Targets on Detection ofReports of biochemistry & molecular biology · 2020Article
- Methods for rapid diagnosis of meningitis etiology in adults.Biomarkers in medicine · 2020Review
- Detection of pathogenic microorganisms from bloodstream infection specimens using TaqMan array card technology.Scientific reports · 2018Article
- Clinical Value of Assessing Cytokine Levels for the Differential Diagnosis of Bacterial Meningitis in a Pediatric Population.Medicine · 2016Observational
- Underestimation of Invasive Meningococcal Disease in Italy.Emerging infectious diseases · 2016Article
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBacterial meningitis is more common in the neonatal period than any other time in life; however, it is still a challenge for the evidence based diagnosis. Strategy for identification of neonatal bacterial meningitis pathogens is presented by evaluating three different available methods to establish evidence-based diagnosis for neonatal bacterial meningitis.
methodsThe cerebrospinal fluid samples from 56 neonates diagnosed as bacterial meningitis in 2009 in Beijing Children's Hospital were analyzed in the study. Two PCR based molecular assays, real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR based-reverse line blot hybridization (mPCR/RLB), were used to assess 7 common neonatal meningitis bacterial pathongens, including Escherichia coli, Staphylococcus aureus, Listerisa monocytogenes, Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, and Streptococcus agalactiae. The findings in examinations of two assays were compared with the results obtained bacterial culture tests.
resultsBacterial meningitis was identified in five cases (9%) by CSF cultures, 25 (45%) by RT-PCR and 16 (29%) by mPCR/RLB. One strain of S. epidermidis and one of E. faecalis were identified using mPCR/RLB but not by RT-PCR. In contrast, cultures identified one strain of S. pneumoniae which was missed by both PCR assays. Overall, the bacterial pathogens in 28 cases were identified with these three methods. Both RT-PCR and mPCR/RLB assays were more sensitive than bacterial culture, (p < 0.05).
conclusionOur study confirmed that both RT-PCR and mPCR/RLB assays have better sensitivity than bacterial culture. They are capable of detecting the pathogens in CSF samples with negative culture results.
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