ArticlePloS one2014
Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 83 citations in OpenAlex.
- Selection and Validation of Stable Reference Genes for RT-qPCR inInternational journal of molecular sciences · 2026Article
- The transformer gene controls sexual development in Drosophila suzukii.Insect science · 2026Article
- Evaluating Real-Time PCR to QuantifyInsects · 2026Article
- Development of an eco-friendly RNAi yeast attractive targeted sugar bait that silences the shaker gene in spotted-wing drosophila, Drosophila suzukii.Pest management science · 2026Article
- Establishment of transgenic Drosophila suzukii lines that express phiC31 integrase and carry the sepia gene as a marker for transformation.Insect molecular biology · 2025Article
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- Short-Term Evolutionary Features and Circadian Clock-Modulated Gene Expression Analysis ofInsects · 2025Article
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- Selection and Validation of Reference Genes for Quantitative Real-Time PCR Analysis in Cockroach ParasitoidInsects · 2024Article
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- Identification of specific reference gene for normalization of RT-qPCR data in rhythmic gene expression studies of the effect of developmental hormone antagonist in postembryonic development inFrontiers in insect science · 2024Article
- Towards a Sustainable Management of the Spotted-Wing Drosophila: Disclosing the Effects of Two Spider Venom Peptides onInsects · 2023Article
- Drosophila suzukii energetic pathways are differently modulated by nutritional geometry in males and females.Scientific reports · 2022Article
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- Identification and Validation of Reference Genes for Gene Expression Analysis inFrontiers in physiology · 2022Article
- Molecular and behavioral studies reveal differences in olfaction between winter and summer morphs ofPeerJ · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To accurately evaluate gene expression levels and obtain more accurate quantitative real-time RT-PCR (qRT-PCR) data, normalization relative to reliable reference gene(s) is required. Drosophila suzukii, is an invasive fruit pest native to East Asia, and recently invaded Europe and North America, the stability of its reference genes have not been previously investigated. In this study, ten candidate reference genes (RPL18, RPS3, AK, EF-1β, TBP, NADH, HSP22, GAPDH, Actin, α-Tubulin), were evaluated for their suitability as normalization genes under different biotic (developmental stage, tissue and population), and abiotic (photoperiod, temperature) conditions. The three statistical approaches (geNorm, NormFinder and BestKeeper) and one web-based comprehensive tool (RefFinder) were used to normalize analysis of the ten candidate reference genes identified α-Tubulin, TBP and AK as the most stable candidates, while HSP22 and Actin showed the lowest expression stability. We used three most stable genes (α-Tubulin, TBP and AK) and one unstably expressed gene to analyze the expression of P-glycoprotein in abamectin-resistant and sensitive strains, and the results were similar to reference genes α-Tubulin, TBP and AK, which show good stability, while the result of HSP22 has a certain bias. The three validated reference genes can be widely used for quantification of target gene expression with qRT-PCR technology in D.suzukii.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.