ArticleInternational journal of genomics2014
Changes in oleic Acid content of transgenic soybeans by antisense RNA mediated posttranscriptional gene silencing.
Article in International journal of genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 54 citations in OpenAlex.
- Changes in Seed Oil Profile and Morphological Characteristics in Sunflower Cultivars Under Salinity Stress and Nanoparticle Treatments.Food science & nutrition · 2025Article
- Identification of SNPs associated with fatty acid contents in mutant soybean lines by a genome-wide association study.Genes & genomics · 2025Article
- KeyInternational journal of molecular sciences · 2023Article
- Development of a High Oleic Cardoon Cell Culture Platform byFrontiers in plant science · 2022Article
- Towards a Cardoon (International journal of molecular sciences · 2021Article
- Enhanced tolerance to Phytophthora root and stem rot by over-expression of the plant antimicrobial peptide CaAMP1 gene in soybean.BMC genetics · 2020Article
- CRISPR-Cas9 mediated targeted disruption of FAD2-2 microsomal omega-6 desaturase in soybean (Glycine max.L).BMC biotechnology · 2019Article
- Over-expression of the Pseudomonas syringae harpin-encoding gene hrpZm confers enhanced tolerance to Phytophthora root and stem rot in transgenic soybean.Transgenic research · 2018Article
- Improved oil quality in transgenic soybean seeds by RNAi-mediated knockdown of GmFAD2-1B.Transgenic research · 2018Article
- RNAi-mediated SMV P3 cistron silencing confers significantly enhanced resistance to multiple Potyvirus strains and isolates in transgenic soybean.Plant cell reports · 2018Article
- Biofortified Crops Generated by Breeding, Agronomy, and Transgenic Approaches Are Improving Lives of Millions of People around the World.Frontiers in nutrition · 2018Review
- Characterization of theFrontiers in plant science · 2017Article
- Direct stacking of sequence-specific nuclease-induced mutations to produce high oleic and low linolenic soybean oil.BMC plant biology · 2016Article
- Significant increase of oleic acid level in the wild species Lepidium campestre through direct gene silencing.Plant cell reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Delta-12 oleate desaturase gene (FAD2-1), which converts oleic acid into linoleic acid, is the key enzyme determining the fatty acid composition of seed oil. In this study, we inhibited the expression of endogenous Delta-12 oleate desaturase GmFad2-1b gene by using antisense RNA in soybean Williams 82. By employing the soybean cotyledonary-node method, a part of the cDNA of soybean GmFad2-1b 801 bp was cloned for the construction of a pCAMBIA3300 vector under the soybean seed promoter BCSP. Leaf painting, LibertyLink strip, PCR, Southern blot, qRT-PCR, and fatty acid analysis were used to detect the insertion and expression of GmFad2-1b in the transgenic soybean lines. The results indicate that the metabolically engineered plants exhibited a significant increase in oleic acid (up to 51.71%) and a reduction in palmitic acid (to <3%) in their seed oil content. No structural differences were observed between the fatty acids of the transgenic and the nontransgenic oil extracts.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.