ArticleJournal of nucleic acids investigation2013
Practical strategies for the evaluation of high-affinity protein/nucleic acid interactions.
Article in Journal of nucleic acids investigation, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Macromolecular High-Affinity Binding Probed by Advanced Fluorescence Techniques.Chembiochem : a European journal of chemical biology · 2025Review
- UV damage induces production of mitochondrial DNA fragments with specific length profiles.Genetics · 2024Article
- The RNA-Binding Domain of hnRNP U Extends beyond the RGG/RG Motifs.Biochemistry · 2024Article
- A high-throughput search for intracellular factors that affect RNA folding identifiesRNA biology · 2024Article
- The Thermodynamic Fingerprints of Ultra-Tight Nanobody-Antigen Binding Probed via Two-Color Single-Molecule Coincidence Detection.International journal of molecular sciences · 2023Article
- UV damage induces production of mitochondrial DNA fragments with specific length profiles.bioRxiv : the preprint server for biology · 2023Article
- Characterization of epitranscriptome reader proteins experimentally andComputational and structural biotechnology journal · 2023Review
- Mechanistic insights into the nickel-dependent allosteric response of the Helicobacter pylori NikR transcription factor.The Journal of biological chemistry · 2023Article
- Analysis of the DNA-binding properties of Alx1, an evolutionarily conserved regulator of skeletogenesis in echinoderms.The Journal of biological chemistry · 2021Article
- How to measure and evaluate binding affinities.eLife · 2020Article
- The glucocorticoid receptor DNA-binding domain recognizes RNA hairpin structures with high affinity.Nucleic acids research · 2019Article
- Combinatorial recognition of clustered RNA elements by the multidomain RNA-binding protein IMP3.Nature communications · 2019Article
- Horizontal Agarose Gel Mobility Shift Assay for Protein-RNA Complexes.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Measuring Low-Picomolar Apparent Binding Affinities by Minigel Electrophoretic Mobility Shift.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Factors affecting the association of single- and double-stranded RNAs with montmorillonite nanoclays.International journal of biological macromolecules · 2018Article
- Human CST Prefers G-Rich but Not Necessarily Telomeric Sequences.Biochemistry · 2017Article
- Rapid agarose gel electrophoretic mobility shift assay for quantitating protein: RNA interactions.Analytical biochemistry · 2016Article
- STN1 OB Fold Mutation Alters DNA Binding and Affects Selective Aspects of CST Function.PLoS genetics · 2016Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The quantitative evaluation of binding interactions between proteins and nucleic acids is highly sensitive to a variety of experimental conditions. Optimization of these conditions is critical for obtaining high quality, reproducible data, particularly in the context of very high affinity interactions. Here, we discuss the practical considerations involved in optimizing the apparent binding constant of an interaction as measured by two common quantitative assays, electrophoretic mobility shift assay and double-filter binding when measuring extremely tight protein/nucleic acid interactions with sub-nanomolar binding affinities. We include specific examples from two telomere end-binding protein systems,
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