ArticleProceedings of the National Academy of Sciences of the United States of America2014
Density-based separation in multiphase systems provides a simple method to identify sickle cell disease.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 1 of them a synthesis that pooled it.
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Who cites it
43 citing papers in PubMed, 1 synthesis or guideline pooled it, 123 citations in OpenAlex.
- Sustainability of newborn screening for sickle cell disease in resource-poor countries: A systematic review.PloS one · 2024Pooled it
- Solvent Interaction Analysis: A New Lens for Protein Structure and Diagnostics.International journal of molecular sciences · 2026Review
- Dissolved Oxygen-Dependent Magnetophoresis of Sickle Red Blood Cells and Proof-of-Concept Separation Using a Magnetic Column.ACS measurement science au · 2026Article
- Acoustic probing of new biomarkers for rapid sickle cell disease screening.Lab on a chip · 2026Article
- Systematic point-of-care newborn screening for sickle cell disease in rural Mali, West Africa.British journal of haematology · 2025Article
- Diagnostic accuracy of HemoTypeSC for detecting sickle cell disease in newborns: A multicentric study.The Indian journal of medical research · 2025Article
- Exploring the limits of magnetic levitation: submicron particle separation and density profiling.Materials research express · 2025Article
- Differential sensitivity to hypoxia enables shape-based classification of sickle cell disease and trait blood samples at point of care.Bioengineering & translational medicine · 2024Article
- The Unique Magnetic Signature of Sickle Red Blood Cells: A Comparison Between the Red Blood Cells of Transfused and Non-Transfused Sickle Cell Disease Patients and Healthy Donors.IEEE transactions on bio-medical engineering · 2022Article
- Translating diagnostics and drug delivery technologies to low-resource settings.Science translational medicine · 2022Review
- Evaluation of Paper-Based Point of Care Screening Test for Sickle Cell Disease.Indian journal of clinical biochemistry : IJCB · 2022Article
- Size and density measurements of single sickle red blood cells using microfluidic magnetic levitation.Lab on a chip · 2022Article
- Using dried blood spot on HemoTypeSC™, a new frontier for newborn screening for sickle cell disease in Nigeria.Frontiers in genetics · 2022Article
- Point-of-care microchip electrophoresis for integrated anemia and hemoglobin variant testing.Lab on a chip · 2021Article
- A portable impedance microflow cytometer for measuring cellular response to hypoxia.Biotechnology and bioengineering · 2021Article
- The flow of sickle blood in glass capillaries: Fundamentals and potential applications.Biophysical journal · 2021Article
- Techniques for the Detection of Sickle Cell Disease: A Review.Micromachines · 2021Review
- Feasibility Study of the "HemoTypeSC" Test for the Rapid Screening of Sickle Cell Disease in Côte D'Ivoire.Advances in hematology · 2021Article
- Dynamics of Individual Red Blood Cells Under Shear Flow: A Way to Discriminate Deformability Alterations.Frontiers in physiology · 2021Article
- Current modalities of sickle cell disease management.Blood science (Baltimore, Md.) · 2020Review
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10 authors at 4 institutions in 1 country.
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Abstract
Although effective low-cost interventions exist, child mortality attributable to sickle cell disease (SCD) remains high in low-resource areas due, in large part, to the lack of accessible diagnostic methods. The presence of dense (ρ > 1.120 g/cm(3)) cells is characteristic of SCD. The fluid, self-assembling step-gradients in density created by aqueous multiphase systems (AMPSs) identifies SCD by detecting dense cells. AMPSs separate different forms of red blood cells by density in a microhematocrit centrifuge and provide a visual means to distinguish individuals with SCD from those with normal hemoglobin or with nondisease, sickle-cell trait in under 12 min. Visual evaluation of a simple two-phase system identified the two main subclasses of SCD [homozygous (Hb SS) and heterozygous (Hb SC)] with a sensitivity of 90% (73-98%) and a specificity of 97% (86-100%). A three-phase system identified these two types of SCD with a sensitivity of 91% (78-98%) and a specificity of 88% (74-98%). This system could also distinguish between Hb SS and Hb SC. To the authors' knowledge, this test demonstrates the first separation of cells by density with AMPSs, and the usefulness of AMPSs in point-of-care diagnostic hematology.
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