ArticleProceedings of the National Academy of Sciences of the United States of America2014
Noncanonical SMC protein in Mycobacterium smegmatis restricts maintenance of Mycobacterium fortuitum plasmids.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 70 citations in OpenAlex.
- UsingACS synthetic biology · 2025Article
- Ultra-high field strength electroporation enables efficient DNA transformation and genome editing in nontuberculous mycobacteria.Microbiology spectrum · 2025Article
- SMC-like Wadjet system prevents plasmid transfer into Clostridium cellulovorans.Applied microbiology and biotechnology · 2025Article
- The extended mobility of plasmids.Nucleic acids research · 2025Review
- Mechanism of DNA capture by the MukBEF SMC complex and its inhibition by a viral DNA mimic.Cell · 2025Article
- Loop-extrusion-mediated plasmid DNA cleavage by the bacterial SMC Wadjet complex.Molecular cell · 2025Article
- MksB is a novel mycobacterial condensin that orchestrates spatiotemporal positioning of replication machinery.Scientific reports · 2024Article
- Loop extrusion-mediated plasmid DNA cleavage by the bacterial SMC Wadjet complex.bioRxiv : the preprint server for biology · 2024Article
- Depletion of tRNA CCA-adding enzyme in Mycobacterium tuberculosis leads to polyadenylation of transcripts and precursor tRNAs.Scientific reports · 2023Article
- SMC-based immunity against extrachromosomal DNA elements.Biochemical Society transactions · 2023Article
- Structural and functional basis of the universal transcription factor NusG pro-pausing activity in Mycobacterium tuberculosis.Molecular cell · 2023Article
- The MksG nuclease is the executing part of the bacterial plasmid defense system MksBEFG.Nucleic acids research · 2023Article
- Mycobacterium smegmatis: The Vanguard of Mycobacterial Research.Journal of bacteriology · 2023Review
- Mycobacterial IHF is a highly dynamic nucleoid-associated protein that assists HupB in organizing chromatin.Frontiers in microbiology · 2023Article
- The SMC-family Wadjet complex protects bacteria from plasmid transformation by recognition and cleavage of closed-circular DNA.Molecular cell · 2022Article
- A joint-ParB interface promotes Smc DNA recruitment.Cell reports · 2022Article
- PADLOC: a web server for the identification of antiviral defence systems in microbial genomes.Nucleic acids research · 2022Article
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- A Comparative Analysis ofInternational journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
8 authors at 6 institutions in 2 countries.
Funding
Abstract
Research on tuberculosis and leprosy was revolutionized by the development of a plasmid transformation system in the fast-growing surrogate, Mycobacterium smegmatis. This transformation system was made possible by the successful isolation of a M. smegmatis mutant strain mc(2)155, whose efficient plasmid transformation (ept) phenotype supported the replication of Mycobacterium fortuitum pAL5000 plasmids. In this report, we identified the EptC gene, the loss of which confers the ept phenotype. EptC shares significant amino acid sequence homology and domain structure with the MukB protein of Escherichia coli, a structural maintenance of chromosomes (SMC) protein. Surprisingly, M. smegmatis has three paralogs of SMC proteins: EptC and MSMEG_0370 both share homology with Gram-negative bacterial MukB; and MSMEG_2423 shares homology with Gram-positive bacterial SMCs, including the single SMC protein predicted for Mycobacterium tuberculosis and Mycobacterium leprae. Purified EptC was shown to bind ssDNA and stabilize negative supercoils in plasmid DNA. Moreover, an EptC-mCherry fusion protein was constructed and shown to bind to DNA in live mycobacteria, and to prevent segregation of plasmid DNA to daughter cells. To our knowledge, this is the first report of impaired plasmid maintenance caused by a SMC homolog, which has been canonically known to assist the segregation of genetic materials.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.