ArticleProceedings of the National Academy of Sciences of the United States of America2014
Combined fluorescent and electron microscopic imaging unveils the specific properties of two classes of meiotic crossovers.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 71 citations in OpenAlex.
- Structure and function of the synaptonemal complex.The Journal of cell biology · 2026Review
- Crossover interference mediates multiscale patterning along meiotic chromosomes.Nature communications · 2025Article
- The Density of Recombination-Associated Genomic Features Does Not Generally Explain the Broad-Scale Crossover Patterns in Chicken and Guinea Fowl.Animals : an open access journal from MDPI · 2025Article
- A Method of Well-Spread Pachytene Chromosome Preparations for Plant Species with Large Genomes Suitable for the Immunolocalization of Meiotic Proteins.Methods and protocols · 2025Article
- Interference length reveals regularity of crossover placement across species.Nature communications · 2024Article
- Meiosis through three centuries.Chromosoma · 2024Review
- Epigenetic regulation during meiosis and crossover.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
- The regulation of meiotic crossover distribution: a coarse solution to a century-old mystery?Biochemical Society transactions · 2023Review
- The Control of the Crossover Localization in Allium.International journal of molecular sciences · 2023Article
- Technology-driven approaches for meiosis research in tomato and wild relatives.Plant reproduction · 2023Article
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- Fanconi anemia ortholog FANCM regulates meiotic crossover distribution in plants.Plant physiology · 2021Article
- Distal Bias of Meiotic Crossovers in Hexaploid Bread Wheat Reflects Spatio-Temporal Asymmetry of the Meiotic Program.Frontiers in plant science · 2021Article
- Exo1 recruits Cdc5 polo kinase to MutLγ to ensure efficient meiotic crossover formation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
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- Sex in Symbiodiniaceae dinoflagellates: genomic evidence for independent loss of the canonical synaptonemal complex.Scientific reports · 2020Review
- MLH1 focus mapping in the guinea fowl (Numida meleagris) give insights into the crossover landscapes in birds.PloS one · 2020Article
- Crossover Interference: Shedding Light on the Evolution of Recombination.Annual review of genetics · 2019Review
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Crossovers (COs) shuffle genetic information and allow balanced segregation of homologous chromosomes during the first division of meiosis. In several organisms, mutants demonstrate that two molecularly distinct pathways produce COs. One pathway produces class I COs that exhibit interference (lowered probability of nearby COs), and the other pathway produces class II COs with little or no interference. However, the relative contributions, genomic distributions, and interactions of these two pathways are essentially unknown in nonmutant organisms because marker segregation only indicates that a CO has occurred, not its class type. Here, we combine the efficiency of light microscopy for revealing cellular functions using fluorescent probes with the high resolution of electron microscopy to localize and characterize COs in the same sample of meiotic pachytene chromosomes from wild-type tomato. To our knowledge, for the first time, every CO along each chromosome can be identified by class to unveil specific characteristics of each pathway. We find that class I and II COs have different recombination profiles along chromosomes. In particular, class II COs, which represent about 18% of all COs, exhibit no interference and are disproportionately represented in pericentric heterochromatin, a feature potentially exploitable in plant breeding. Finally, our results demonstrate that the two pathways are not independent because there is interference between class I and II COs.
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