ArticleInternational journal of molecular sciences2014
In vitro selection of a single-stranded DNA molecular recognition element against atrazine.
Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Selection, characterization, and biosensing applications of DNA aptamers targeting cyanotoxin BMAA.RSC advances · 2024Article
- Combating small molecule environmental contaminants: detection and sequestration using functional nucleic acids.Chemical science · 2022Review
- Effective Electrochemiluminescence Aptasensor for Detection of Atrazine Residue.Sensors (Basel, Switzerland) · 2022Article
- One-step simultaneous quantitative detection of three pesticides based on bimetallic organic framework nanomaterials and aptamers.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2022Article
- Translational control of enzyme scavenger expression with toxin-induced micro RNA switches.Scientific reports · 2021Article
- Pesticide Aptasensors-State of the Art and Perspectives.Sensors (Basel, Switzerland) · 2020Review
- An overview and future prospects on aptamers for food safety.Applied microbiology and biotechnology · 2020Review
- In-Solution Molecular Recognition Comparison of Aptamers against the Herbicide Atrazine.ACS omega · 2019Article
- In Vitro Selection and Characterization of a Single-Stranded DNA Aptamer Against the Herbicide Atrazine.ACS omega · 2018Article
- Isolation of DNA aptamers for herbicides under varying divalent metal ion concentrations.Aptamers (Oxford, England) · 2018Article
- In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against the Pesticide Fipronil and Sensitive Detection in River Water.International journal of molecular sciences · 2017Article
- Small-Molecule Binding Aptamers: Selection Strategies, Characterization, and Applications.Frontiers in chemistry · 2016Review
- Capillary electrophoresis applied to DNA: determining and harnessing sequence and structure to advance bioanalyses (2009-2014).Analytical and bioanalytical chemistry · 2015Review
- In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serum.International journal of molecular sciences · 2015Article
- Single-Stranded DNA Aptamers against Pathogens and Toxins: Identification and Biosensing Applications.BioMed research international · 2015Review
- In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Clostridium difficile Toxin B and Sensitive Detection in Human Fecal Matter.Journal of nucleic acids · 2015Article
- Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human Serum.BioMed research international · 2015Article
- In vitro selection of a single-stranded DNA molecular recognition element specific for bromacil.Journal of nucleic acids · 2014Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Widespread use of the chlorotriazine herbicide, atrazine, has led to serious environmental and human health consequences. Current methods of detecting atrazine contamination are neither rapid nor cost-effective. In this work, atrazine-specific single-stranded DNA (ssDNA) molecular recognition elements (MRE) were isolated. We utilized a stringent Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology that placed the greatest emphasis on what the MRE should not bind to. After twelve rounds of SELEX, an atrazine-specific MRE with high affinity was obtained. The equilibrium dissociation constant (Kd) of the ssDNA sequence is 0.62 ± 0.21 nM. It also has significant selectivity for atrazine over atrazine metabolites and other pesticides found in environmentally similar locations and concentrations. Furthermore, we have detected environmentally relevant atrazine concentrations in river water using this MRE. The strong affinity and selectivity of the selected atrazine-specific ssDNA validated the stringent SELEX methodology and identified a MRE that will be useful for rapid atrazine detection in environmental samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.