ArticleActa crystallographica. Section F, Structural biology communications2014
Microseed matrix screening for optimization in protein crystallization: what have we learned?
Article in Acta crystallographica. Section F, Structural biology communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed.
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- Crystal Structure and Conformational Dynamics of N─N Bond-Forming Piperazate Synthase.Chembiochem : a European journal of chemical biology · 2026Article
- Crystallization and 1.6 Å resolution crystal structure of an acylated GLP-1/GIP analogue peptide.Acta crystallographica. Section F, Structural biology communications · 2026Article
- X-ray analysis of complexes of carbonic anhydrase II with 1,3-oxazole-containing sulfonamide derivatives elucidates the structural basis for their exceptionally high inhibitory potency.Acta crystallographica. Section F, Structural biology communications · 2026Article
- Molecular structure and nickel-binding capacity of Proteus mirabilis UreE.Acta crystallographica. Section D, Structural biology · 2026Article
- Automated microbatch-under-oil phase diagrams to rationalize serial crystallography sample preparation.IUCrJ · 2026Article
- The Starting Point for Biomolecular Crystallisation.Advances in biochemical engineering/biotechnology · 2026Review
- Biomolecular Crystallisation Through Soft Templates and Seeding.Advances in biochemical engineering/biotechnology · 2026Review
- Alternative Strategies to Promote Biomolecule Nucleation.Advances in biochemical engineering/biotechnology · 2026Review
- Programming Defects and Cavities into Colloidal Crystals Engineered With DNA.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Preparing for successful protein crystallization experiments.Acta crystallographica. Section F, Structural biology communications · 2025Article
- Efficient Crystallization of Apo Sirt2 for Small-Molecule Soaking and Structural Analysis of Ligand Interactions.Journal of medicinal chemistry · 2025Article
- Structures of α-galactosaminidases from the CAZy GH114 family and homologs defining a new GH191 family of glycosidases.Acta crystallographica. Section D, Structural biology · 2025Article
- Review
- A generic cross-seeding approach to protein crystallization.Journal of applied crystallography · 2025Article
- Revealing protein structures: crystallization of protein-ligand complexes - co-crystallization and crystal soaking.FEBS open bio · 2025Article
- Human dystrophin tandem calponin homology actin-binding domain crystallized in a closed-state conformation.Acta crystallographica. Section D, Structural biology · 2025Article
- Expansion of the diversity of dispersin scaffolds.Acta crystallographica. Section D, Structural biology · 2025Article
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- Far-reaching effects of tyrosine64 phosphorylation on Ras revealed with BeFCommunications chemistry · 2024Article
11 more citing papers are in PubMed but not listed here.
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4 authors.
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Abstract
Protein crystals obtained in initial screens typically require optimization before they are of X-ray diffraction quality. Seeding is one such optimization method. In classical seeding experiments, the seed crystals are put into new, albeit similar, conditions. The past decade has seen the emergence of an alternative seeding strategy: microseed matrix screening (MMS). In this strategy, the seed crystals are transferred into conditions unrelated to the seed source. Examples of MMS applications from in-house projects and the literature include the generation of multiple crystal forms and different space groups, better diffracting crystals and crystallization of previously uncrystallizable targets. MMS can be implemented robotically, making it a viable option for drug-discovery programs. In conclusion, MMS is a simple, time- and cost-efficient optimization method that is applicable to many recalcitrant crystallization problems.
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