ArticleExperimental animals2015
Single-step generation of rabbits carrying a targeted allele of the tyrosinase gene using CRISPR/Cas9.
Article in Experimental animals, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 69 citations in OpenAlex.
- A Novel Anti-Cancer Therapy: CRISPR/Cas9 Gene Editing.Frontiers in pharmacology · 2022Review
- CRISPR/Cas9-Mediated Genetic Engineering to Generate a Disease Model Prairie Vole, Based on Species-Optimized Assisted Reproductive Technology.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Analysis ofAnimals : an open access journal from MDPI · 2021Article
- Genetically Modified Rabbits for Cardiovascular Research.Frontiers in genetics · 2021Review
- Generation of knockout rabbits with X-linked severe combined immunodeficiency (X-SCID) using CRISPR/Cas9.Scientific reports · 2020Article
- Genome engineering technologies in rabbits.Journal of biomedical research · 2020Article
- ZP4 confers structural properties to the zona pellucida essential for embryo development.eLife · 2019Article
- Strategies to reduce genetic mosaicism following CRISPR-mediated genome edition in bovine embryos.Scientific reports · 2019Article
- CRISPR-Cas9 Gene Editing in Lizards through Microinjection of Unfertilized Oocytes.Cell reports · 2019Article
- Efficient derivation of knock-out and knock-in rats using embryos obtained by in vitro fertilization.Scientific reports · 2019Article
- Article
- Broad-Spectrum and Personalized Guide RNAs for CRISPR/Cas9 HIV-1 Therapeutics.AIDS research and human retroviruses · 2018Article
- Generation of genetically-engineered animals using engineered endonucleases.Archives of pharmacal research · 2018Review
- Directions and applications of CRISPR technology in livestock research.Animal reproduction · 2018Article
- A novel rabbit model of Duchenne muscular dystrophy generated by CRISPR/Cas9.Disease models & mechanisms · 2018Article
- Production of Wilson Disease Model Rabbits with Homology-Directed Precision Point Mutations in the ATP7B Gene Using the CRISPR/Cas9 System.Scientific reports · 2018Article
- CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering.Genetics research international · 2018Review
- Rabbit models for biomedical research revisited via genome editing approaches.The Journal of reproduction and development · 2017Review
- Naming CRISPR alleles: endonuclease-mediated mutation nomenclature across species.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- CRISPR/Cas9 Immune System as a Tool for Genome Engineering.Archivum immunologiae et therapiae experimentalis · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Targeted genome editing of nonrodent mammalian species has provided the potential for highly accurate interventions into gene function in humans and the generation of useful animal models of human diseases. Here we show successful clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated (Cas)-mediated gene targeting via circular plasmid injection in rabbits. The rabbit tyrosinase gene (TYR) was effectively disrupted, and we confirmed germline transmission by pronuclear injection of a circular plasmid expressing humanized Cas9 (hCas9) and single-guide RNA. Direct injection into pronuclear stage zygotes was possible following an in vitro validation assay. Neither off-target mutagenesis nor hCas9 transgenesis was detected in any of the genetically targeted pups and embryos examined. Gene targeting with this rapid and simplified strategy will help accelerate the development of translational research using other nonrodent mammalian species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.